AI Article Synopsis
- Spermatogonial stem cells (SSCs), which produce sperm in the testes, can be transformed into germline-derived pluripotent stem (gPS) cells while retaining their androgenetic imprinting patterns.* ! -
- The study explored whether these imprinting patterns change during the differentiation of gPS cells into neural stem cells (gPS-NSCs), finding that certain patterns remained stable while others were altered.* ! -
- The findings indicate that there are epigenetic changes in genomic imprinting during the process, highlighting the potential of gPS-NSCs as a model for investigating imprinted genes in brain development and neurodevelopmental disorders.* !
Article Abstract
Spermatogonial stem cells (SSCs), which are unipotent stem cells in the testes that give rise to sperm, can be converted into germline-derived pluripotent stem (gPS) by self-induction. The androgenetic imprinting pattern of SSCs is maintained even after their reprogramming into gPS cells. In this study, we used an in vitro neural differentiation model to investigate whether the imprinting patterns are maintained or altered during differentiation. The androgenetic patterns of H19, Snrpn, and Mest were maintained even after differentiation of gPS cells into NSCs (gPS-NSCs), whereas the fully unmethylated status of Ndn in SSCs was altered to somatic patterns in gPS cells and gPS-NSCs. Thus, our study demonstrates epigenetic alteration of genomic imprinting during the induction of pluripotency in SSCs and neural differentiation, suggesting that gPS-NSCs can be a useful model to study the roles of imprinted genes in brain development and human neurodevelopmental disorders.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854545 | PMC |
| http://dx.doi.org/10.1080/15592294.2016.1146852 | DOI Listing |
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