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High-throughput imaging method for direct assessment of GM1 ganglioside levels in mammalian cells. | LitMetric

High-throughput imaging method for direct assessment of GM1 ganglioside levels in mammalian cells.

Data Brief

BioStrategies LC, State University, P.O. Box 2428, AR 72467, USA; Arkansas Biosciences Institute & Department Biological Sciences, Arkansas State University, P.O. Box 639, Jonesboro, AR 72467, USA.

Published: March 2016

AI Article Synopsis

Article Abstract

GM1-gangliosidosis is an inherited autosomal recessive disorder caused by mutations in the gene GLB1, which encodes acid β-galactosidase (β-gal). The lack of activity in this lysosomal enzyme leads to accumulation of GM1 gangliosides (GM1) in cells. We have developed a high-content-imaging method to assess GM1 levels in fibroblasts that can be used to evaluate substrate reduction in treated GLB1(-/-) cells [1]. This assay allows fluorescent quantification in a multi-well system which generates unbiased and statistically significant data. Fluorescently labeled Cholera Toxin B subunit (CTXB), which specifically binds to GM1 gangliosides, was used to detect in situ GM1 levels in a fixed monolayer of fibroblasts. This sensitive, rapid, and inexpensive method facilitates in vitro drug screening in a format that allows a high number of replicates using low working volumes.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763105PMC
http://dx.doi.org/10.1016/j.dib.2016.01.027DOI Listing

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