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A novel and low-cost cross-priming amplification assay for rapid detection of Babesia duncani infection.
Exp Parasitol
October 2024
State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address:
Babesia duncani, responsible for human babesiosis, is one of the most important tick-borne intraerythrocytic pathogens. Traditionally, babesiosis is definitively diagnosed by detecting parasite DNA in blood samples and examining Babesia parasites in Giemsa-stained peripheral blood smears. Although these techniques are valuable for determining Babesia duncani, they are often time-consuming and laborious.
View Article and Find Full Text PDFIdentifying suitable habitat for Ixodes scapularis (Acari: Ixodidae) infected with Anaplasma phagocytophilum (Rickettsiales: Anaplasmataceae), Babesia microti (Piroplasmida: Babesiidae), and Borrelia miyamotoi (Spirochaetales: Spirochaetaceae) to guide surveillance efforts in the eastern United States.
J Med Entomol
May 2023
Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Pr evention, Fort Collins, CO 80521, USA.
Understanding the distribution of infected ticks is informative for the estimation of risk for tickborne diseases. The blacklegged tick, Ixodes scapularis (Acari: Ixodidae), is the primary vector for 7 medically significant pathogens in United States. However, knowledge of the ranges of these pathogens in host-seeking ticks is incomplete, particularly for those occurring at low prevalence.
View Article and Find Full Text PDFParasitologist-level classification of apicomplexan parasites and host cell with deep cycle transfer learning (DCTL).
Bioinformatics
August 2020
College of Science, Harbin Institute of Technology, Shenzhen, China.
Motivation: Apicomplexan parasites, including Toxoplasma, Plasmodium and Babesia, are important pathogens that affect billions of humans and animals worldwide. Usually a microscope is used to detect these parasites, but it is difficult to use microscopes and clinician requires to be trained. Finding a cost-effective solution to detect these parasites is of particular interest in developing countries, in which infection is more common.
View Article and Find Full Text PDFPrevention of transfusion-transmitted infections.
Blood
April 2019
Department of Pathology, University of British Columbia, Victoria, BC, Canada.
Since the 1970s, introduction of serological assays targeting virus-specific antibodies and antigens has been effective in identifying blood donations infected with the classic transfusion-transmitted infectious agents (TTIs; hepatitis B virus [HBV], HIV, human T-cell lymphotropic virus types I and II, hepatitis C virus [HCV]). Subsequently, progressive implementation of nucleic acid-amplification technology (NAT) screening for HIV, HCV, and HBV has reduced the residual risk of infectious-window-period donations, such that per unit risks are <1 in 1 000 000 in the United States, other high-income countries, and in high-incidence regions performing NAT. NAT screening has emerged as the preferred option for detection of newer TTIs including West Nile virus, Zika virus (ZIKV), and Although there is continual need to monitor current risks due to established TTI, ongoing challenges in blood safety relate primarily to surveillance for emerging agents coupled with development of rapid response mechanisms when such agents are identified.
View Article and Find Full Text PDFCost-effectiveness of a Babesia microti blood donation intervention based on real-time prospective screening in endemic areas of the United States.
Transfusion
March 2016
American Red Cross, Scientific Support Office, Gaithersburg, MD.
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