AI Article Synopsis
- The research confirms that KCa3.1 (IKCa) channels are present in CA1 hippocampal pyramidal cells and play a role in regulating neuron behavior through a slow afterhyperpolarization.
- The study employs single cell RT-PCR to identify mRNA sequences related to the IKCa channel, highlighting key binding sites for four specific blockers.
- It also discusses challenges in effectively applying the IKCa blocker TRAM-34 internally, noting that starting the drug treatment at the beginning of experiments is not feasible due to rapid drug action after being introduced for whole-cell recordings.
Article Abstract
Our previous work reported that KCa3.1 (IKCa) channels are expressed in CA1 hippocampal pyramidal cells and contribute to the slow afterhyperpolarization that regulates spike accommodation in these cells. The current report presents data from single cell RT-PCR that further reveals mRNA in CA1 cells that corresponds to the sequence of an IKCa channel from transmembrane segments 5 through 6 including the pore region, revealing the established binding sites for 4 different IKCa channel blockers. A comparison of methods to internally apply the IKCa channel blocker TRAM-34 shows that including the drug in an electrode from the onset of an experiment is unviable given the speed of drug action upon gaining access for whole-cell recordings. Together the data firmly establish IKCa channel expression in CA1 neurons and clarify methodological requirements to obtain a block of IKCa channel activity through internal application of TRAM-34.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4954577 | PMC |
| http://dx.doi.org/10.1080/19336950.2016.1161988 | DOI Listing |
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