This study aimed to investigate the fatigue effects induced by a futsal-specific protocol (FIRP) on sprint performance and the kinematics of the lower limbs. Twenty-one futsal players participated in this study and performed a protocol to simulate the futsal demands. At pre-protocol, half-time and post-protocol, the athletes performed 10-m sprints that were recorded for kinematic analysis. Continuous relative phase (CRP) was calculated to assess the inter-segmental coordination. In addition, vertical (K) and leg (K) stiffness were calculated. Analysis of variance (ANOVA) for repeated measures was used (P < 0.05). The main results showed that sprint time increased (P < 0.01) post-protocol when compared to pre- and half-time conditions. Lower values of the step rate (P = 0.01) and higher values of the leg angular velocity (P = 0.02) were verified at the end of the FIRP. The CRP of thigh-leg and leg-foot and the stiffness did not change over the protocol. In addition, the high correlation of CRP between the conditions revealed no changes in coordination pattern. We concluded that futsal related-fatigue induced a decrement on sprint time, changing the kinematics of the lower limbs (decreasing step rate and increasing leg angular velocity). However, neither stiffness nor intersegment coordination during sprints was affected by fatigue.

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