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Development of a fast DNA extraction method for sea food and marine species identification. | LitMetric

Development of a fast DNA extraction method for sea food and marine species identification.

Food Chem

National Research Council - Institute for Coastal Marine Environment, UOS Capo Granitola, Laboratory of Molecular Ecology and Biotechnology, Via del Faro n. 3, 91021 Torretta Granitola, Trapani, Sicily, Italy. Electronic address:

Published: July 2016

AI Article Synopsis

Article Abstract

The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis.

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Source
http://dx.doi.org/10.1016/j.foodchem.2016.02.095DOI Listing

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