Gastro-intestinal exclusion by Roux-en-Y gastric bypass (RYGB) improves glucose metabolism, independent of weight loss. Although changes in intestinal bile trafficking have been shown to play a role, the underlying mechanisms are unclear. We performed RYGB in minipigs and showed that the intestinal uptake of ingested glucose is blunted in the bile-deprived alimentary limb (AL). Glucose uptake in the AL was restored by the addition of bile, and this effect was abolished when active glucose intestinal transport was blocked with phlorizin. Sodium-glucose cotransporter 1 remained expressed in the AL, while intraluminal sodium content was markedly decreased. Adding sodium to the AL had the same effect as bile on glucose uptake. It also increased postprandial blood glucose response in conscious minipigs following RYGB. The decrease in intestinal uptake of glucose after RYGB was confirmed in humans. Our results demonstrate that bile diversion affects postprandial glucose metabolism by modulating sodium-glucose intestinal cotransport.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.cmet.2016.01.018 | DOI Listing |
J Virol
January 2025
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Porcine epidemic diarrhea virus (PEDV), as a type of Alphacoronavirus causing acute diarrhea and high death rate among sucking piglets, poses great financial damage to the swine industry. Nevertheless, the molecular mechanism whereby PEDV enters host cells is unclear, limiting the development of PED vaccines and anti-PEDV agents. The present study found that the host protein ribonuclease kappa (RNASEK) was regulated by USF2, a transcription factor, and facilitated the PEDV replication.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, PR China, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China. Electronic address:
In recent years, the toxicity of microplastics (MPs) in combination with heavy metals, particularly the influence of varying microplastic sizes on their toxic effects, has attracted widespread attention. In this study, red swamp crayfish (Procambarus clarkia) were exposed to MPs of two particle sizes (S-MPs: 5 μm, 1 mg/L; and L-MPs: 100 μm, 1 mg/L) and Cu (5 mg/L) individually or in combination for 96 h. The accumulation patterns of MPs were as follows: gills > intestines > hepatopancreas > muscles.
View Article and Find Full Text PDFEur J Med Chem
January 2025
Forschungszentrum Jülich GmbH, Institute of Neuroscience and Medicine, Nuclear Chemistry (INM-5), Wilhelm-Johnen-Straße, 52428, Jülich, Germany; University of Cologne, Faculty of Medicine and University Hospital Cologne, Institute of Radiochemistry and Experimental Molecular Imaging, Kerpener Straße 62, 50937, Cologne, Germany.
Serotonergic 5-HT receptors in the cortex and other forebrain structures have been linked to cognitive, emotional and memory processes. In addition, dysfunction or altered expression of these receptors is associated with neuropsychiatric and neurodegenerative disorders. [F]R91150 is a candidate radiotracer for positron emission tomography (PET) imaging of 5-HT receptors, which showed promising properties in in vitro studies.
View Article and Find Full Text PDFJ Comp Physiol B
January 2025
Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Elasmobranchs are commonly carnivores and are important in energy transfer across marine ecosystems. Despite this, relatively few studies have examined the physiological underpinnings of nutrient acquisition in these animals. Here, we investigated the mechanisms of uptake at the spiral valve intestine for two representative amino acids (-alanine, -leucine) and one representative fatty acid (oleic acid), each common to the diet of a carnivore, the Pacific spiny dogfish (Squalus suckleyi).
View Article and Find Full Text PDFBiomater Sci
January 2025
Engineered Living Materials Institute, Cornell University, Ithaca, NY 14850, USA.
An increase in plastic waste and its release into the environment has led to health concerns over microplastics (MPs) in the environment. The intestinal mucosal layer is a key defense mechanism against ingested MPs, preventing the migration of particles to other parts of the body. MP migration through intestinal mucus is challenging to study due to difficulties in obtaining intact mucus layers for testing and numerous formulations, shapes, and sizes of microplastics.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!