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Antioxidant mito-TEMPO prevents the increase in tropomyosin oxidation and mitochondrial calcium accumulation under 7-day rat hindlimb suspension.
Free Radic Biol Med
November 2024
Institute of Biomedical Problems, RAS, Moscow, Russia. Electronic address:
After the first day of muscle disuse (unloading) mitochondria-derived ROS accumulate in the postural-tonic soleus muscle. It is known that excess of ROS can lead to the accumulation of intramitochondrial calcium and overload of mitochondria with calcium, can negatively affect mitochondrial function and fatigue resistance of soleus muscle. We assumed that the use of mitochondrial ROS scavenger mito-TEMPO will be able to prevent the unloading-induced disruption of mitochondrial functions and will help maintain soleus muscle fatigue resistance.
View Article and Find Full Text PDFTwo-dimensional photonic crystal acetylcholinesterase hydrogel and organohydrogel sensors for efficient detection of organophosphorus compounds.
Biosens Bioelectron
January 2025
Key Laboratory of New Energy & New Functional Materials, Shaanxi Key Laboratory of Chemical Reaction Engineering, College of Chemistry and Chemical Engineering, Yan'an University, Yan'an, Shaanxi, 716000, China. Electronic address:
Sensors capable of detecting organophosphorus (OP) compounds have attracted the most attention owing to severe OP contamination worldwide. Despite many years of research, the developed OP sensors mainly focused on detecting water-soluble OPs in proper environments and the exploration of OP sensors suitable in resource-limited areas is extremely challenging. Here, a simple two-dimensional photonic crystal (2D PC) hydrogel featuring capabilities of effectively quantitative determination of OP compounds is facilely constructed by immobilizing the enzyme acetylcholinesterase (AChE) onto a bovine serum albumin (BSA) protein hydrogel.
View Article and Find Full Text PDFSelf-Assembly-Activated Engineered Magnetic Biohybrids Loaded with Phosphotriesterase for Sustainable Decontamination and Detection of Organophosphorus Pesticides.
J Agric Food Chem
October 2024
State Key Laboratory of NBC Protection for Civilian, Research Institute of Chemical Defense, Academy of Military Science, Beijing 102205, China.
Phosphotriesterase (PTE) biodegradation of organophosphorus pesticides (OPs) is an efficient and environmentally friendly method. However, the instability and nonreusability of free PTE become the key factors restricting its practical application. In this study, a novel cross-linked magnetic hybrid nanoflower (CLMNF) was prepared.
View Article and Find Full Text PDFA Portable Electrochemical Biosensor Based on an Amino-Modified Ionic Metal-Organic Framework for the One-Site Detection of Multiple Organophosphorus Pesticides.
ACS Appl Mater Interfaces
October 2024
Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, PR China.
Constructing stable, portable sensors and revealing their mechanisms is challenging. Ion metal-organic frameworks (IMOFs) are poised to serve as highly effective electrochemical sensors for detecting organophosphorus pesticides (OPs), leveraging their unique charge properties. In this work, an amino-modified IMOF was constructed and combined with near-field communication (NFC) technology to develop a portable, touchless, and battery-free electrochemical biosensor .
View Article and Find Full Text PDFTuning the Properties of Polyvinylidene Fluoride/Alkali Lignin Membranes to Develop a Biocatalytic Membrane Reactor for an Organophosphorus Pesticide Degradation.
Membranes (Basel)
August 2024
National Research Council of Italy, Institute on Membrane Technology, CNR-ITM, 87036 Rende, Italy.
It has been observed that the immobilization of a phosphotriesterase enzyme (PTE) onto polyvinylidene fluoride (PVDF) membranes significantly decreased the enzyme activity, and this negative effect was attributed to the hydrophobic character of the membrane. The indirect indication of this reason was that the same enzyme immobilized on other membrane materials bearing hydrophilic character showed better performance. In this work, we provide direct evidence of the mechanism by immobilizing a PTE on a PVDF membrane hydrophilized by blending it with alkali lignin (AL).
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