AI Article Synopsis
- Most patients with acute falciparum malaria develop antibodies against the circumsporozoite (CS) protein, which is crucial for immune response.
- A study involving 13 Thai patients tracked their antibody responses using two methods: an indirect fluorescent antibody test and an enzyme-linked immunosorbent assay (ELISA).
- The results indicated that antibody responses were consistent across both methods, and the use of specific peptide constructs can accurately measure anti-sporozoite antibodies regardless of the infection stage or past malaria experience.
Article Abstract
Most acute falciparum malaria patients mount an antibody response to the circumsporozoite (CS) protein which contains a dominant B-cell epitope. In order to investigate whether antibodies against other epitopes on the sporozoite surface may be important during a particular phase of infection or convalescence, we longitudinally studied the antibody responses of 13 Thai patients with acute falciparum malaria. Antibody comparisons were made using intact Plasmodium falciparum sporozoites in an indirect fluorescent antibody test and the recombinant peptide, R32tet32, as capture antigen in an enzyme-linked immunosorbent assay. Antibody response curves derived using the 2 methods were similar, and adsorption with R32tet32 greatly (greater than 95%) diminished anti-sporozoite activity in sera. Thus, peptide constructs containing the CS repeat region epitope, (NANP)n, can be used with confidence to assay anti-sporozoite antibodies, independent of both the time of infection and prior malaria history.
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| http://dx.doi.org/10.1016/0035-9203(89)90623-8 | DOI Listing |
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