AI Article Synopsis
- 3D cell culture models serve as a bridge between animal testing and traditional 2D cultures, providing a more accurate representation of human biology.
- Previous studies using MCF-7 human breast cancer cells in a scaffold-free 3D system showed that exposure to estradiol impacted lumen formation and altered cell morphology and gene expression related to estrogen signaling.
- The differentiated 3D culture system enables researchers to explore specific phenotypic and molecular changes that result from exposure to various estrogenic compounds.
Article Abstract
In the development of human cell-based assays, 3-dimensional (3D) cell culture models are intriguing as they are able to bridge the gap between animal models and traditional two-dimensional (2D) cell culture. Previous work has demonstrated that MCF-7 human breast carcinoma cells cultured in a 3D scaffold-free culture system self-assemble and develop into differentiated microtissues that possess a luminal space. Exposure to estradiol for 7 days decreased lumen formation in MCF-7 microtissues, altered microtissue morphology and altered expression of genes involved in estrogen signaling, cell adhesion and cell cycle regulation. Exposure to receptor-specific agonists for estrogen receptor alpha, estrogen receptor beta and g-protein coupled estrogen receptor resulted in unique, receptor-specific phenotypes and gene expression signatures. The use of a differentiated scaffold-free 3D culture system offers a unique opportunity to study the phenotypic and molecular changes associated with exposure to estrogenic compounds.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803074 | PMC |
| http://dx.doi.org/10.1016/j.toxlet.2016.02.012 | DOI Listing |
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