JIP3 and JIP4, two highly related scaffolding proteins for MAP kinases, are binding partners for two molecular motors as well as for the small G protein ARF6. The leucine zipper II (LZII) region of JIP3/4 is the binding site for these three partners. Previously, the crystal structure of ARF6 bound to JIP4 revealed LZII in a parallel coiled-coil arrangement. Here, the crystal structure of an N-terminally truncated form of LZII of JIP3 alone shows an unexpected antiparallel arrangement. Using molecular dynamics and modelling, the stability of this antiparallel LZII arrangement, as well as its specificity for ARF6, were investigated. This study highlights that N-terminal truncation of LZII can change its coiled-coil orientation without affecting its overall stability. Further, a conserved buried asparagine residue was pinpointed as a possible structural determinant for this dramatic structural rearrangement. Thus, LZII of JIP3/4 is a versatile structural motif, modifications of which can impact partner recognition and thus biological function.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4774878 | PMC |
| http://dx.doi.org/10.1107/S2053230X16001576 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Accelerated Low-Dose Total Skin Electron Beam Therapy Using the Modified Stanford Technique: An In Vivo Dosimetry Confirmation Study.
Cureus
December 2024
Radiation Oncology, Thomas Jefferson University Hospital, Philadelphia, USA.
Purpose Low-dose total skin electron beam therapy (LD-TSEBT) has recently gained popularity in treating mycosis fungoides (MF) due to its reduced toxicity and favorable response rates. Combining accelerated LD-TSEBT with the modified Stanford technique (mST), a condensed cycling approach, offers a promising and convenient option. However, in vivo dosimetry data confirming the effectiveness of this approach is limited.
View Article and Find Full Text PDFOptical soliton solutions of the M-fractional paraxial wave equation.
Sci Rep
January 2025
Department of Mathematics, Pabna University of Science and Technology, Pabna, 6600, Bangladesh.
This research used a modified and extended auxiliary mapping method to examine the optical soliton solutions of the truncated time M-fractional paraxial wave equation. We employed the truncated time M-fractional derivative to eliminate the fractional order in the governing model. The few optical wave examples of the paraxial wave condition can assume an insignificant part in depicting the elements of optical soliton arrangements in optics and photonics for the investigation of different actual cycles, including the engendering of light through optical frameworks like focal points, mirrors, and fiber optics.
View Article and Find Full Text PDFThe monoclonal antibody 17E1 selectively labels GFAP in 5xFAD mice.
Alzheimers Dement
December 2024
University of Kentucky College of Medicine, Sanders‐Brown Center on Aging, Lexington, KY, USA
Background: Our lab recently developed 2 mouse monoclonal antibodies that preferentially react with “distressed astrocytes”. One monoclonal, 26A6, was found to react preferentially with a form of the Ca2+/calmodulin‐dependent protein phosphatase, calcineurin (CN), that has been cleaved by calpain, to generate a 48 kDa CN fragment (∆CN). We recently published a characterization of this antibody.
View Article and Find Full Text PDFUnlocking the potential of β-limit dextrin: Preparation, structure, properties, and promising applications.
Carbohydr Polym
March 2025
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Institute of Future Food Technology, JITRI, Yixing 214200, China. Electronic address:
Starch is a widely used and economically important polymer; however, its industrial applications are limited by certain shortcomings, such as retrogradation and high digestion rate. To overcome these limitations, native starches can be hydrolyzed by β-amylase, resulting in the production of β-limit dextrin (β-LD) and maltose as a co-product. β-LD retains the original inner core structure of its parent amylopectin and contains truncated external chains that is not prone to form exterior chain helical.
View Article and Find Full Text PDFTopologies of Nanoscale Droplets upon Head-On Collision from Large Molecular Dynamics Simulations.
Langmuir
January 2025
Thermodynamik, Technische Universität Berlin, 10587 Berlin, Germany.
The binary collision of nanoscale droplets is studied with molecular dynamics simulation for droplets consisting of up to 2 × 10 molecules interacting via a truncated and shifted form of the Lennard-Jones potential. Considering head-on collisions of droplets with a temperature near the triple point that occur in a saturated vapor of the same fluid, this work explores a range of collision topologies. Four droplet sizes, with a radius ranging from 30 to 120 molecule diameters, are simulated with a varying initial relative collision velocity, covering 36 cases in total.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!