AI Article Synopsis
- The study explores the largely uncharted territory of mitochondrial DNA (mtDNA) cytosine methylation (5 mC) and its implications for endothelial cell (EC) aging and cardiovascular health.
- There is a significant discovery of unique clusters of 5 mC and an uneven distribution of methylation across mtDNA non-coding regions in senescent EC.
- Findings suggest that these methylation patterns, particularly hypo-methylation at replication regulatory sites, may mainly influence D-loop formation rather than directly affecting mitochondrial gene expression.
Article Abstract
The regulation and function of Mitochondrial DNA (mtDNA) cytosine methylation (5 mC) are largely unexplored. Mitochondria, Endothelial Cell (EC) senescence, and cardiovascular dysfunction are closely related. We extensively investigated the mtDNA Non-Coding Region (NCR) methylation pattern and its variations in EC replicative senescence. We observed previously undescribed 5 mC clusters and a biased distribution of 5 mC among DNA sites and throughout the NCR. The methylation pattern in senescent EC showed non-random variations, including the hypo-methylation of mtDNA replication regulatory sites. Additional experiments opened to a possible role for 5 mC in D-loop formation, rather than in mitochondrial gene expression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mito.2016.02.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!