The polar hydroethanolic extract from Selaginella sellowii(SSPHE) has been previously proven active on intracellular amastigotes (in vitro test) and now was tested on hamsters infected with Leishmania (Leishmania) amazonensis (in vivo test). SSPHE suppressed a 100% of the parasite load in the infection site and draining lymph nodes at an intralesional dose of 50 mg/kg/day × 5, which was similar to the results observed in hamsters treated with N-methylglucamine antimonate (Sb) (28 mg/Kg/day × 5). When orally administered, SSPHE (50 mg/kg/day × 20) suppressed 99.2% of the parasite load in infected footpads, while Sb suppressed 98.5%. SSPHE also enhanced the release of nitric oxide through the intralesional route in comparison to Sb. The chemical fingerprint of SSPHE by high-performance liquid chromatography with diode-array detection and tandem mass spectrometry showed the presence of biflavonoids and high molecular weight phenylpropanoid glycosides. These compounds may have a synergistic action in vivo. Histopathological study revealed that the intralesional treatment with SSPHE induced an intense inflammatory infiltrate, composed mainly of mononuclear cells. The present findings reinforce the potential of this natural product as a source of future drug candidates for American cutaneous leishmaniasis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804496 | PMC |
| http://dx.doi.org/10.1590/0074-02760150307 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Antidiabetic and Antigout Properties of the Ultrasound-Assisted Extraction of Total Biflavonoids from Revealed by In Vitro and In Silico Studies.
Antioxidants (Basel)
September 2024
School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.
In this study, the extraction, purification and metabolic enzyme inhibition potential of were investigated. In order to extract the total biflavonoids from (SDTBs), the optimum extraction process was obtained by optimizing the ultrasonic extraction parameters using response-surface methodology. This resulted in a total biflavonoid content of 22.
View Article and Find Full Text PDFIntegrating computational methods and experimental validation reveals the pharmacological mechanism of (L.) Baker targeting major proteins in breast cancer.
Heliyon
October 2024
Department of Biology, College of Science, University of Ha'il, Ha'il, P.O. Box 2440, Saudi Arabia.
Breast cancer remains a significant global health challenge, necessitating the exploration of novel therapeutic options. The present study employs an integrated approach encompassing network pharmacology, molecular docking, molecular dynamics simulations, and validation to investigate the potential of in breast cancer treatment. Initial network pharmacology analysis revealed different potential targets and pathways associated with breast cancer that could be modulated by phytochemical constituents.
View Article and Find Full Text PDFAmentoflavone maintaining extracellular matrix homeostasis and inhibiting subchondral bone loss in osteoarthritis by inhibiting ERK, JNK and NF-κB signaling pathways.
J Orthop Surg Res
October 2024
Department of Orthopedics, Nanchang Hongdu Hospital of TCM, Nanchang, China.
Amentoflavone (AF), a plant biflavone isolated from Selaginella sinensis ethanol extract, is characterized by anti-inflammatory and anti-oxidant properties. According to previous studies, inflammation and oxidative stress are closely related to the pathophysiology of osteoarthritis (OA). However, the effects and mechanisms of AF on OA have not been elucidated.
View Article and Find Full Text PDFIntegration strategies involving 16S rDNA sequencing combined with untargeted metabolomics revealed the mechanism of Selaginella tamariscina (Beauv.) Spring in db/db diabetic mice.
Biomed Pharmacother
November 2024
Henan University of Chinese Medicine, 156 Jinshui East Road, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, 156 Jinshui East Road, Zhengzhou 450046, China. Electronic address:
Selaginella tamariscina extracts (STS), total flavonoids of Selaginella tamariscina (TFST) and the main active component amentoflavone (AMT) have hypoglycaemic-mitigating effects, but their efficacy and mechanism of action in db/db mice are unknown. This study aimed to evaluate the hypoglycaemic effects of Selaginella tamariscina and its extracts in db/db diabetic mice and explore their mechanisms through gut microbiota modulation and metabolomics. Sixty male db/db mice were divided into model (db/db), STS (1.
View Article and Find Full Text PDFAmentoflavone Induces Ferroptosis to Alleviate Proliferation, Migration, Invasion and Inflammation in Rheumatoid Arthritis Fibroblast-like Synoviocytes by Inhibiting PIN1.
Cell Biochem Biophys
October 2024
Department of Pharmacy, Beijing Chaoyang Hospital, Capital Medical University, 100020, Beijing, People's Republic of China.
Article Synopsis
- Rheumatoid arthritis (RA) is a serious disease that affects many people worldwide, and special cells called RA-FLS are important in its progression.
- A study tested a natural compound called Amentoflavone (AMF) to see how it could affect these RA-FLS cells.
- The results showed that AMF can slow down harmful behaviors of these cells, like growing and spreading too much, by targeting a protein called PIN1 and causing a process called ferroptosis, which could help in treating RA.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!