AI Article Synopsis
- The study investigates how DNA induces the aggregation of gold nanoparticles (GNPs) using advanced imaging techniques like laser scanning confocal microscopy and environmental scanning electron microscopy.
- Observations reveal that the fluorescence shifts and broadens as GNPs aggregate, indicating Förster resonance energy transfer occurs when a hydrophobic probe interacts with surfactant-coated GNPs.
- The results show that DNA can organize GNPs into 3D structures resembling the DNA helix, providing insights for developing improved nanobiotechnology applications.
Article Abstract
The gold nanoparticle (GNP) aggregation growth induced by deoxyribonucleic acid (DNA) is studied by laser scanning confocal and environmental scanning electron microscopies. As in the investigated case the direct light scattering analysis is not suitable, we observe the behavior of the fluorescence produced by a dye and we detect the aggregation by the shift and the broadening of the fluorescence peak. Results of laser scanning confocal microscopy images and the fluorescence emission spectra from lambda scan mode suggest, in fact, that the intruding of the hydrophobic moiety of the probe within the cationic surfactants bilayer film coating GNPs results in a Förster resonance energy transfer. The environmental scanning electron microscopy images show that DNA molecules act as template to assemble GNPs into three-dimensional structures which are reminiscent of the DNA helix. This study is useful to design better nanobiotechnological devices using GNPs and DNA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801634 | PMC |
| http://dx.doi.org/10.3390/s16020258 | DOI Listing |
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