Neurotransmission at dopaminergic synapses has been studied with techniques that provide high temporal resolution, but cannot resolve individual synapses. To elucidate the spatial dynamics and heterogeneity of individual dopamine boutons, we developed fluorescent false neurotransmitter 200 (FFN200), a vesicular monoamine transporter 2 (VMAT2) substrate that selectively traces monoamine exocytosis in both neuronal cell culture and brain tissue. By monitoring electrically evoked Ca(2+) transients with GCaMP3 and FFN200 release simultaneously, we found that only a small fraction of dopamine boutons that exhibited Ca(2+) influx engaged in exocytosis, a result confirmed with activity-dependent loading of the endocytic probe FM1-43. Thus, only a low fraction of striatal dopamine axonal sites with uptake-competent VMAT2 vesicles are capable of transmitter release. This is consistent with the presence of functionally 'silent' dopamine vesicle clusters and represents, to the best of our knowledge, the first report suggestive of presynaptically silent neuromodulatory synapses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4853199 | PMC |
| http://dx.doi.org/10.1038/nn.4252 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Lysosome-targeted dual-locked NIR fluorescent probe for visualization of HS and viscosity in drug-induced liver injury and tumor models.
Anal Chim Acta
February 2025
Department of Radiology, Changhai Hospital, Naval Medical University, Changhai Road 168, Shanghai 200433, China. Electronic address:
Background: Lysosomes, as an indispensable subcellular organelle have numerous physiological functions closely associated with HS and viscosity, and accurate assessment of HS/viscosity fluctuations in lysosomes is essential for gaining a comprehensive understanding of lysosome-related physiological activities and pathological processes. The previous single-response fluorescent probes for either HS or viscosity alone have the potential to generate "false positive" signals in a complex biological environment. In contrast, dual-locked probes can simultaneously respond to multiple targets simultaneously, which could effectively eliminate this defect.
View Article and Find Full Text PDFTemplate-assembly activation of primer exchange reaction for on-site and sensitive detection of copper ions in blood.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, 250355, PR China; Department of Pharmacy, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, PR China. Electronic address:
The sensitive and accurate detection of copper ions is crucial for public health, medical research, and environmental monitoring. In this study, we developed a sensor based on template-assembly activation of the primer exchange reaction (PER) for the on-site detection of copper ions in blood. Copper ions triggered the assembly of two template fragments into a hairpin structure via a click-chemistry reaction, activating the PER.
View Article and Find Full Text PDFHistology and Lung Nodule Fluorescence in Intraoperative Molecular Imaging With Pafolacianine.
Ann Thorac Surg Short Rep
September 2024
Division of Thoracic Surgery, Department of Surgery, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania.
Background: Intraoperative molecular imaging (IMI) uses a cancer-targeted fluorescent agent injected into patients to localize tumor nodules. Pafolacianine is a folate receptor (FR)-targeted near-infrared fluorescent probe. Almost 10% of patients have false negative fluorescence findings intraoperatively.
View Article and Find Full Text PDF3D Imaging Resolves Human Pancreatic Duct-β-Cell Clusters During Cystic Change.
Diabetes
January 2025
Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan.
Pancreatic cystic changes in adults are increasingly identified through advanced cross-sectional imaging. However, the impact of initial/intra-lobular epithelial remodeling on the local β-cell population remains unclear. In this study, we examined 10 human cadaveric donor pancreases (tail and body regions) via integration of stereomicroscopy, clinical H&E histology, and 3D immunohistochemistry, identifying 36 microcysts (size: 1.
View Article and Find Full Text PDFAdvanced Dual-Mode Microfluidic Sensing Platform Based on Amphiphilic Polymer-Capped Perovskite Nanozymes Induced Photoelectrochemical Signal Amplification and Fluorescence Emission.
Anal Chem
January 2025
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
A novel dual-mode microfluidic sensing platform integrating photoelectrochemical (PEC) and fluorescence (FL) sensors was developed for the sensitive monitoring of heart fatty acid binding protein (h-FABP). First, BiVO/AgInS (BVAIS) composites with excellent photoelectric activity were synthesized as sensing matrices. The BVAIS heterojunction with a well-matched internal energy level structure provided a stable photocurrent.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!