AI Article Synopsis
- Cancer cells produce high amounts of lactate, which is linked to higher chances of metastasis and tumor regrowth, making it crucial to study.
- A new droplet microfluidic method enables rapid and precise measurement of lactate release from individual cancer cells by using fluorescence to indicate lactate levels.
- This technique allows researchers to analyze variations in lactate release among different cancer cell lines, potentially aiding in cancer biology studies and understanding cell metabolism more generally.
Article Abstract
Cancer cells release high levels of lactate that has been correlated to increased metastasis and tumor recurrence. Single-cell measurements of lactate release can identify malignant cells and help decipher metabolic cancer pathways. We present here a novel droplet microfluidic method that allows the fast and quantitative determination of lactate release in many single cells. Using passive forces, droplets encapsulated cells are positioned in an array. The single-cell lactate release rate is determined from the increase in droplet fluorescence as the lactate is enzymatically converted to a fluorescent product. The method is used to measure the cell-to-cell variance of lactate release in K562 leukemia and U87 glioblastoma cancer cell lines and under the chemical inhibition of lactate efflux. The technique can be used in the study of cancer biology, but more broadly in cell biology, to capture the full range of stochastic variations in glycolysis activity in heterogeneous cell populations in a repeatable and high-throughput manner.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813300 | PMC |
| http://dx.doi.org/10.1021/acs.analchem.5b04681 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Targeting Non-Apoptotic Pathways with the Cell Permeable TAT-Conjugated NOTCH1 RAM Fragment for Leukemia and Lymphoma Cells.
ACS Omega
December 2024
Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan.
Targeting nonapoptotic cell death offers a promising strategy for overcoming apoptosis resistance in cancer. In this study, we developed Tat-Ram13, a 25-mer peptide that fuses the NOTCH1 intracellular domain fragment RAM13 with a cell-penetrating HIV-1 TAT, for the treatment of T-cell acute lymphoblastic leukemia with aberrant NOTCH1 mutation. Tat-Ram13 significantly downregulated NOTCH1-target genes in T-ALL cell lines.
View Article and Find Full Text PDFProtective effect of Auraptene, a novel acetylcholinesterase inhibitor, on hydrogen peroxide-induced cell toxicity in PC12 cells.
Toxicol Res (Camb)
December 2024
Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Khorasan Razavi, Mashhad, Azadi Square, 9177948954, Iran.
Objective: Alzheimer's disease (ad) is a progressive and degenerative disorder of the central nervous system that is associated with cognitive and memory impairment. The main factors which have been implicated in neurodegeneration of ad are oxidative stress and cholinergic neurons dysfunction. Here, we examined the effects of auraptene, a novel acetylcholinesterase (AChE) inhibitor, on hydrogen peroxide (HO)-induced cell death in PC12 cells.
View Article and Find Full Text PDFFolic Acid-Decorated Chitosan-PLGA Nanobiopolymers for Targeted Drug Delivery to Acute Lymphoblastic Leukemia Cells: Studies.
Technol Cancer Res Treat
December 2024
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Objectives: This study developed a drug delivery system (DDS) using folic acid (FA)-functionalized chitosan (CS) and poly (lactic-co-glycolic acid) (PLGA) nanocarriers for targeted sodium butyrate (NB) delivery to leukemia cells (NALM6). The goal was to enhance NB's therapeutic efficacy while reducing its cytotoxicity to non-malignant cells.
Methods: FA-CS-PLGA nanocarriers were synthesized and characterized using Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta potential analysis, transmission electron microscopy (TEM), and thermogravimetric analysis (TGA).
Preparation of bioactive film for regulating chlorine dioxide release based on the hygroscopic properties of chitosan and its application in broccoli preservation.
Int J Biol Macromol
December 2024
Department of Packaging Engineering, Jiangnan University, No.1800 Lihu Avenue, Wuxi, Jiangsu 214122, China; Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment & Technology, Wuxi, Jiangsu 214122, China.
An active packaging film was developed by integrating sodium chlorite (SC) and citric acid (CA) into a Poly(lactic acid)/Poly(butylene adipate-co-terephthalate) (PLA/PBAT) matrix, enabling the controlled release of chlorine dioxide (ClO) gas. The release of ClO was further regulated by introducing chitosan (CS) into the film, leveraging its hygroscopic properties. The results showed that when the addition amount of CS was 4 wt%, the water vapor transmission rate increased by 41.
View Article and Find Full Text PDFSyringable Microcapsules for Sustained, Localized, and Controllable siRNA Delivery.
ACS Appl Mater Interfaces
December 2024
School of Engineering and Applied Sciences (SEAS), Harvard University, Cambridge, Massachusetts 02138, United States.
The clinical use of small interfering RNA (siRNA) and antisense oligonucleotides often requires invasive routes of administration, including intrathecal or intraocular injection. Additionally, these treatments often necessitate repeated injections. While nanoparticle formulation and chemical modifications have extended siRNA therapeutic durability, challenges persist, such as the side effects of bolus injections with high toxicity and maximum exposure in the acute phase.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!