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A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells. | LitMetric

AI Article Synopsis

  • Long noncoding RNAs, like SENCR, are important in regulating stem cell differentiation and understanding their role in endothelial biology is still developing.
  • SENCR is linked to the FLI1 gene, which is crucial for endothelial development, and our research showed that it influences the commitment of cells to mesodermal and endothelial lineages.
  • We found that while SENCR does not affect the ability of cells to remain pluripotent, its overexpression boosts early development into endothelial cells and enhances functions like cell proliferation and migration in human umbilical endothelial cells, with altered expression patterns observed in patients with vascular diseases.

Article Abstract

Despite the increasing importance of long noncoding RNA in physiology and disease, their role in endothelial biology remains poorly understood. Growing evidence has highlighted them to be essential regulators of human embryonic stem cell differentiation. SENCR, a vascular-enriched long noncoding RNA, overlaps the Friend Leukemia Integration virus 1 (FLI1) gene, a regulator of endothelial development. Therefore, we wanted to test the hypothesis that SENCR may contribute to mesodermal and endothelial commitment as well as in endothelial function. We thus developed new differentiation protocols allowing generation of endothelial cells from human embryonic stem cells using both directed and hemogenic routes. The expression of SENCR was markedly regulated during endothelial commitment using both protocols. SENCR did not control the pluripotency of pluripotent cells; however its overexpression significantly potentiated early mesodermal and endothelial commitment. In human umbilical endothelial cell (HUVEC), SENCR induced proliferation, migration, and angiogenesis. SENCR expression was altered in vascular tissue and cells derived from patients with critical limb ischemia and premature coronary artery disease compared to controls. Here, we showed that SENCR contributes to the regulation of endothelial differentiation from pluripotent cells and controls the angiogenic capacity of HUVEC. These data give novel insight into the regulatory processes involved in endothelial development and function.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4876031PMC
http://dx.doi.org/10.1038/mt.2016.41DOI Listing

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