The presence of Polysorbate 80 in samples can challenge liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) analysis as it is easily ionised and detected. In this study, we demonstrate that interference from Polysorbate 80 can be reduced by complexation with a metal ion followed by precipitation by thiocyanate. The precipitation procedure was tested on a mixture of low molecular weight compounds (e.g. amino acids and non-amino organic acids) and it was shown that none of the tested compounds were precipitated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00216-016-9326-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pneumatic tube transport of trastuzumab in IV bags -effect of headspace and surfactant on subvisible particle formation.
J Pharm Sci
January 2025
Department of Process and Life Science Engineering, Div. Food and Pharma, Lund University, P.O. Box 124, 22100 Lund, Sweden.
In hospitals, IV bags can be prepared in advance for logistical and microbial safety reasons in a compounding unit and then transported to wards. Transport of protein drugs using a pneumatic tube system has been reported to result in high particle levels. In this study, pneumatic tube transport of trastuzumab in saline polyolefin bags was compared to delivery by hospital porters using an electric platform truck in an underground tunnel system.
View Article and Find Full Text PDFChallenges with effective removal of surfactants from monoclonal antibody formulations.
Int J Pharm
December 2024
Department of Pharmaceutical Sciences & Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, the United States of America. Electronic address:
Buffer exchange is a critical step in biologics development, playing a pivotal role in removing contaminants, adjusting sample conditions, and facilitating compatibility studies. The efficiency of centrifugal concentrators for polysorbate removal was compared to a two-step approach involving a surfactant removal column followed by buffer exchange. Trastuzumab-pkrb from Herzuma® was used.
View Article and Find Full Text PDFRemediation of a diesel contaminated soil by means of anionic and non-ionic surfactants: Effect on soil phosphorus availability and Vicia Faba L. growth.
Sci Total Environ
January 2025
Department of Engineering, University of Palermo, Viale delle Scienze, building 8, 90128 Palermo, Italy. Electronic address:
In the present study, the effectiveness of two surfactants (Polysorbate 80 - Tween 80 and Sodium Dodecyl Benzensulphonate - SDBS) was investigated for the remediation of a hydrocarbon-contaminated soil. Moreover, it was elucidated the impact of surfactants on soil phosphorus (P) availability and phytotoxic effect on the growth of Vicia Faba L. An experimental laboratory-scale apparatus (bench and pilot scale) was set up for the simulation of a soil flushing intervention.
View Article and Find Full Text PDFEnhancing the biodegradation of hydrophobic volatile organic compounds: A study on microbial consortia adaptation and the role of surfactants.
J Environ Manage
November 2024
Research Group EnVOC, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
The emission of hydrophobic Volatile Organic Compounds (VOCs) is a serious environmental issue. Typically, biofilters (BFs) are employed for their treatment, with the potential enhancement of mass transfer through the addition of surfactants. However, disparate results in previous studies have been observed, attributed to uncontrolled conditions during the introduction of surfactants to BFs.
View Article and Find Full Text PDFIsolation and quantification of human urinary exosomes using a Tween-20 elution solvent from polyester, capillary-channeled polymer fiber columns.
Anal Chim Acta
November 2024
Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, SC, 29634-0973, USA. Electronic address:
Article Synopsis
- Exosomes are crucial for cell communication and have potential roles in therapy, making their isolation and quantification important for research.
- This study evaluates Tween-20 as an elution solvent for exosome recovery, demonstrating its effectiveness in isolating high-purity exosomes quickly.
- The new method incorporates advanced detection techniques, ensuring accurate measurement of exosome concentration and size, which can benefit various fields like clinical diagnostics and biochemistry.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!