Aim: It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research.

Materials & Methods: Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector.

Results & Conclusion: Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.

Download full-text PDF

Source
http://dx.doi.org/10.4155/bio.15.260DOI Listing

Publication Analysis

Top Keywords

vitamin compounds
16
compounds human
8
human blood
8
method vitamin
8
vitamin
5
simultaneous quantitative
4
quantitative analysis
4
analysis vitamin
4
compounds
4
blood lc-ms/ms
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!