AI Article Synopsis
- Inherited ciHHV-6 in hematopoietic cell transplant (HCT) donors or recipients complicates testing for HHV-6 reactivation, which happens in 30-50% of cases.
- A new multiplex droplet digital PCR assay can differentiate between HHV-6 species (A or B) and detect inherited ciHHV-6.
- Testing showed that 25% of HCT recipients with ciHHV-6A experienced reactivation of HHV-6B, highlighting the importance of accurate diagnostic testing for HHV-6 infection despite the presence of ciHHV-6.
Article Abstract
The presence of inherited chromosomally integrated human herpesvirus 6 (ciHHV-6) in hematopoietic cell transplant (HCT) donors or recipients confounds molecular testing for HHV-6 reactivation, which occurs in 30 to 50% of transplants. Here we describe a multiplex droplet digital PCR clinical diagnostic assay that concurrently distinguishes between HHV-6 species (A or B) and identifies inherited ciHHV-6. By applying this assay to recipient post-HCT plasma and serum samples, we demonstrated reactivation of HHV-6B in 25% (4/16 recipients) of HCT recipients with donor- or recipient-derived inherited ciHHV-6A, underscoring the need for diagnostic testing for HHV-6 infection even in the presence of ciHHV-6.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844705 | PMC |
| http://dx.doi.org/10.1128/JCM.03275-15 | DOI Listing |
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