AI Article Synopsis
- A protocol was developed for the efficient micropropagation of Canna indica L. using rhizome explants from aseptic seedlings, leading to high regeneration rates in a specific nutrient medium.
- The ideal conditions included MS medium with 3.0 mg/L BAP and 1.5 mg/L NAA for shoot and root production, and 7.0 mg/L BAP for multiple shoot regeneration.
- After two months of greenhouse acclimatization, the plantlets demonstrated a 75% survival rate, with ultrastructural studies indicating minor developmental differences between in vitro and in vivo specimens.
Article Abstract
An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739482 | PMC |
| http://dx.doi.org/10.1155/2016/2820454 | DOI Listing |
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