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| http://dx.doi.org/10.1073/pnas.1600174113 | DOI Listing |
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Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design.
Front Bioeng Biotechnol
December 2024
AO Vector-Best, Novosibirsk, Russia.
Introduction: Modification of natural enzymes to introduce new properties and enhance existing ones is a central challenge in bioengineering. This study is focused on the development of Taq polymerase mutants that show enhanced reverse transcriptase (RTase) activity while retaining other desirable properties such as fidelity, 5'- 3' exonuclease activity, effective deoxyuracyl incorporation, and tolerance to locked nucleic acid (LNA)-containing substrates. Our objective was to use AI-driven rational design combined with multiparametric wet-lab analysis to identify and validate Taq polymerase mutants with an optimal combination of these properties.
View Article and Find Full Text PDFPhosphite mediated molecular editing switch to -C-H alkylation of isoquinolines: emergence of a distinct photochemical [1,3] N to C rearrangement.
Chem Sci
December 2024
Organic Chemistry Division, CSIR-National Chemical Laboratory (CSIR-NCL) Pune 411 008 India
The isoquinoline core is present in one of the largest subsets of bioactive natural products. The multifunctional isoquinoline core exerts diverse bioactivity, resulting in the development of numerous isoquinoline-based drugs and molecules that are currently under clinical trials. We developed a new approach for phosphite-mediated [1,2] alkyl migration for an overall -C-H alkylation -alkylation of isoquinoline.
View Article and Find Full Text PDFMyringoplasty: A comparison of inlay and underlay techniques.
J Otol
April 2024
Department of Otolaryngology-Head and Neck Surgery, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
Purpose: Myringoplasty aims to improve hearing and prevent otorrhea, making graft uptake rates and hearing crucial considerations. This study analyzed the factors associated with unsuccessful graft uptake and hearing improvement to guide the selection of optimal surgical approaches for myringoplasty.
Methods: We retrospectively reviewed 56 ears with chronic otitis media.
Application of machine learning and neural network models based on experimental evaluation of dissimilar resistance spot-welded joints between grade 2 titanium alloy and AISI 304 stainless steel.
Heliyon
December 2024
Department of Manufacturing Processes and Production Engineering, Rzeszow University of Technology, al. Powst. Warszawy 8, 35-959, Rzeszów, Poland.
The use of a composite welded joint consisting of titanium and austenitic stainless steel metals is evidently a favourable selection for industrial applications employing the resistance spot welding (RSW) operation. Nevertheless, achieving a high-quality welded joint proved challenging owing to the properties of the diverse range of materials' used. To improve the quality of dissimilar welded joints, the welding parameters should be selected precisely.
View Article and Find Full Text PDFRenewable energy transition in Europe in the context of renewable energy transition processes in the world. A review.
Heliyon
December 2024
GPCHEM, Laboratory of Biogas Research and Analysis & Technology Incubator, Ul. Legionów 40a/3, 87-100 Toruń, Poland.
Both the global and European energy sectors have been undergoing a deep transition for several years, associated with a reduction in the overall share of conventional coal-based energy in favor of new technologies, especially energy from renewable sources (RES). This transition is moving from centralized production towards distributed technologies and from providing only energy to end users towards combining innovative products and services with it. Electricity consumers are becoming prosumers connected to the grid and generating an increasing amount of energy.
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