AI Article Synopsis
- A new method for multiplexed protein quantification combines neutron encoding (NeuCode) metabolic labeling and parallel reaction monitoring (PRM) to enhance targeted protein analysis.
- This technique uses high-resolution tandem mass spectrometry to allow for the quantification of multiple NeuCode-labeled peptides, significantly increasing the level of multiplexing.
- The method was successfully applied to track viral infections and protein production in cultured cells, providing up to 30 channels of quantitative data within a single mass spectrometry experiment.
Article Abstract
We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectra. Here we demonstrate this approach in cultured cells by monitoring a viral infection and the corresponding viral protein production over many infection time points in a single experiment. In this context the NeuCode PRM combination affords up to 30 channels of quantitative information in a single MS experiment.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5141612 | PMC |
| http://dx.doi.org/10.1021/acs.analchem.5b04773 | DOI Listing |
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