AI Article Synopsis
- Recent studies show that oxidative RNA damage leads to translation issues and can cause diseases, with 8-hydroxyguanosine (8-oxo-rG) being a key oxidized base.
- Researchers developed a new molecule, 2'-O-methoxy Adap (2'-OMeAdap), to specifically target 8-oxo-rG in RNA, building on previous work with DNA oxidized base 8-oxo-dG.
- The study found that 8-oxo-rG in homopurine RNA could be detected using an ODN probe with Adap, while a different probe using 2'-OMeAdap successfully identified 8-oxo-rG in homopyrimidine RNA despite instability issues
Article Abstract
Recent studies indicate that oxidative damage to RNA results in dysfunction of translation and eventual pathogenesis. A representative oxidized base in RNA is 8-hydroxyguanosine (8-oxo-rG), however, unlike its DNA counterpart (8-oxo-dG), its role in pathogenesis has not attracted much attention until recently. The 2'-deoxyadenosine derivative with a diazaphenoxazine skeleton at the 6-amino group (Adap) was shown to be selective for 8-oxo-dG in DNA. In this study, the 2'-O-methoxy derivative of Adap (2'-OMeAdap) was designed as a selective molecule for 8-oxo-rG in RNA. 8-Oxo-rG in the homopurine RNA was selectively recognized by the ODN probe incorporating Adap. In contrast, although it was not possible by the Adap-containing ODN prove due to the instability of the corresponding duplex, 8-oxo-rG in homopyrimidine RNA was selectively detected by the 2'-OMeRNA probe incorporating 2'-OMeAdap.
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| http://dx.doi.org/10.1016/j.bmc.2016.02.001 | DOI Listing |
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