Objective: This study investigated the anti-demineralizing and antibacterial effects of a propolis ethanolic extract (EEP) against Streptococcus mutans dental biofilm.
Design: Blocks of sound bovine enamel (n=24) were fixed on polystyrene plates. S. mutans inoculum (ATCC 25175) and culture media were added (48 h-37 °C) to form biofilm. Blocks with biofilm received daily treatment (30 μL/1 min), for 5 days, as following: G1 (EEP 33.3%); G2 (chlorhexidine digluconate 0.12%); G3 (ethanol 80%); and G4 (Milli-Q water). G5 and G6 were blocks without biofilm that received only EEP and Milli-Q water, respectively. Final surface hardness was evaluated and the percentage of hardness loss (%HL) was calculated. The EEP extract pH and total solids were determined. S. mutans count was expressed by log10 scale of Colony-Forming Units (CFU/mL). One way ANOVA was used to compare results which differed at a 95% significance level.
Results: G2 presented the lowest average %HL value (68.44% ± 12.98) (p=0.010), while G4 presented the highest (90.49% ± 5.38%HL) (p=0.007). G1 showed %HL (84.41% ± 2.77) similar to G3 (87.80% ± 6.89) (p=0.477). Groups G5 and G6 presented %HL=16.11% ± 7.92 and 20.55% ± 10.65; respectively (p=0.952). G1 and G4 differed as regards to S. mutans count: 7.26 ± 0.08 and 8.29 ± 0.17 CFU/mL, respectively (p=0.001). The lowest bacterial count was observed in chlorhexidine group (G2=6.79 ± 0.10 CFU/mL) (p=0.043). There was no difference between S. mutans count of G3 and G4 (p=0.435). The EEP showed pH 4.8 and total soluble solids content=25.9 Brix.
Conclusion: The EEP seems to be a potent antibacterial substance against S. mutans dental biofilm, but presented no inhibitory action on the de-remineralization of caries process.
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http://dx.doi.org/10.1016/j.archoralbio.2016.02.001 | DOI Listing |
J Dent (Shiraz)
December 2024
Dept. of Prosthodotics, Faculty of Dentistry Sabzevar University of Medical Sciences, Sabzevar, Iran.
Statement Of The Problem: Antimicrobial photodynamic therapy (aPDT) is a protocol proposed for reduction of bacterial load in deep dentin caries in primary and permanent dentitions. However, considering the difference in the morphology of dentinal tubules in primary and permanent teeth, the effect of this treatment may be different on the two dentition types.
Purpose: This systematic review and meta-analysis aimed to assess the effect of type of dentition as a determinant of microbial load reduction by aPDT in deep dentin caries.
J Long Term Eff Med Implants
December 2024
Department of Periodontology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, India.
Although the surface modifications of the implant have been widely proposed to enhance the osseointegration, their impact on microbial adhesion is still not explored. The present in vitro study was done to compare the bacterial adhesion of S. mutans and Enterococcus faecalis on sand-blasted acid-etched and anodized titanium dental implants.
View Article and Find Full Text PDFBiofouling
December 2024
Laboratory of Applied Microbiology Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP), School of Dentistry, Araraquara, SP, Brazil.
The efficacy of Zerumbone (ZER) against mixed biofilms of fluconazole-resistant (ATCC 96901) and (UA159) was evaluated. Biofilms were cultivated on acrylic resin specimens for 48 h, with alternating supplementation of glucose and sucrose. ZER's ability to inhibit biofilm formation (pre-treatment) and eradicate mature biofilms (post-treatment) was assessed.
View Article and Find Full Text PDFJ Int Soc Sports Nutr
December 2025
University "G. D'Annunzio" of Chieti-Pescara, Department of Medical, Oral and Biotechnological Sciences, Chieti, Italy.
Background: Athletes' oral health can impact overall well-being and sports performance. This study aimed to evaluate the interactions between eating habits and oral health of 120 young athletes as compared to 30 age-matched individuals not practicing sports based on a questionnaire and the analysis of saliva.
Methods: One hundred twenty subjects practicing various sports activities (test group) and 30 subjects not practicing sports (control group) were selected.
Clin Exp Dent Res
December 2024
Oral and Dental Diseases Research Center, Department of Pediatrics Dentistry, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Objectives: Lactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S.
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