AI Article Synopsis
- Arabidopsis thaliana is a key model organism used to study the genetic pathways that control seed germination in plants.
- The seed germination process in Arabidopsis includes stages like seed coat rupture, root development, and leaf expansion, which are influenced by environmental factors like light, temperature, and water levels.
- The research also explores the endosperm's role in inhibiting germination and provides methods for assessing the impact of hormones like gibberellin and abscisic acid on the germination process.
Article Abstract
The model organism Arabidopsis thaliana has been extensively used to unmask the molecular genetic signaling pathways controlling seed germination in plants. In Arabidopsis, the normal seed to seedling developmental transition involves testa rupture soon followed by endosperm rupture, radicle elongation, root hair formation, cotyledon expansion, and greening. Here we detail a number of basic procedures to assess Arabidopsis seed germination in response to different light (red and far-red pulses), temperature (seed thermoinhibition), and water potential (osmotic stress) environmental conditions. We also discuss the role of the endosperm and how its germination-repressive activity can be monitored genetically by means of a seed coat bedding assay. Finally we detail how to evaluate germination responses to changes in gibberellin (GA) and abscisic acid (ABA) levels by manipulating pharmacologically the germination medium.
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| http://dx.doi.org/10.1007/978-1-4939-3356-3_15 | DOI Listing |
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