The environmental conditions during culture enrichment, which ultimately determine its maximum specific biodegradation rate (qmax) and affinity for the target pollutant (Ks), play a key role in the performance of bioreactors devoted to the treatment of methane emissions. This study assessed the influence of Cu(2+) and CH4 concentration and the effective CH4 supply rate during culture enrichment on the structure and biodegradation kinetics of methanotrophic communities. The results obtained demonstrated that an increase in Cu(2+) concentration from 0.05 to 25 μM increased the qmax and Ks of the communities enriched by a factor of ≈ 3, even if the Cu(2+) concentration did not seem to have an effect on the enzymatic "copper switch" and only pMMO was detected. In addition, high Cu(2+) concentrations supported lower diversity coefficients (Hs ≈ 1.5× lower) and apparently promoted the growth of more adapted methanotrophs such as Methylomonas. Despite no clear effect of CH4 concentration on the population structure or on the biodegradation kinetics of the communities enriched was recorded at the two low CH4 concentrations studied (1 and 8%), a higher agitation rate increased the qmax by a factor of ≈ 2.3 and Ks by a factor of ≈ 3.1.
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http://dx.doi.org/10.1016/j.jenvman.2016.02.002 | DOI Listing |
Am J Primatol
January 2025
Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, China.
Recent evidence challenging the notion of a sterile intrauterine environment has sparked research into the origins and effects of fetal microbiota on immunity development during gestation. Rhesus macaques (RMs) serve as valuable nonhuman primate models due to their similarities to humans in development, placental structure, and immune response. In this study, metagenomic analysis was applied to the placenta, umbilical cord, spleen, gastrointestinal tissues of an unborn RM fetus, and the maternal intestine, revealing the diversity and functionality of microbes in these tissues.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
Institute for Biomedical Technologies - National Research Council (ITB-CNR), Segrate, Milan, Italy.
Amyloidosis diseases are characterized by protein misfolding, which forms insoluble beta-sheet fibrils progressively deposited in tissues. Deposition in the form of amyloid aggregates can occur in various organs, damaging their structure and function. The hallmark of amyloidosis is aberrant interactions leading to protein aggregation and proteotoxicity.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
University of California - Davis, Department of Environmental Toxicology, Davis, CA, USA.
Hair is a ubiquitous and robust mammalian tissue with biological, clinical, forensic, social, and economic significance. The hair shaft proteome reflects both structural proteins, dominated by cuticular intermediate filament keratins and associated proteins, and proteins involved in the final cellular processes of terminally differentiating corneocytes prior to cornification. These distinct biological processes involve cell maintenance, biosynthesis, senescence, and xenobiotic response.
View Article and Find Full Text PDFNature
December 2024
State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China.
Commun Biol
December 2024
Department of Computer Science, Kent State University, 800 E Summit St, Kent, OH, 44242, USA.
Recent years have witnessed the remarkable progress of deep learning within the realm of scientific disciplines, yielding a wealth of promising outcomes. A prominent challenge within this domain has been the task of predicting enzyme function, a complex problem that has seen the development of numerous computational methods, particularly those rooted in deep learning techniques. However, the majority of these methods have primarily focused on either amino acid sequence data or protein structure data, neglecting the potential synergy of combining both modalities.
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