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Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers. | LitMetric
Context : There is insufficient knowledge about the chondrocyte membranome and its molecular composition.Objective : To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins.Materials And Methods : Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS.Results : A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms.Discussion : Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery.
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