AI Article Synopsis
- The study aimed to assess how transplanted induced pluripotent stem cells (iPSCs) affect functional recovery and protein regulation in a rat model of Huntington's disease (HD).
- After 8 weeks of iPSC transplantation, the rats showed improved motor function and increased glucose metabolism in the affected brain region, confirmed by PET/CT scans.
- Additionally, the transplanted cells migrated to the damaged area and differentiated into relevant neurons, while specific proteins were significantly upregulated, suggesting a positive biochemical response to the treatment.
Article Abstract
Aims: The purpose of this study was to determine the functional recovery and protein regulation by transplanted induced pluripotent stem cells in a rat model of Huntington's disease (HD).
Methods: In a quinolinic acid-induced rat model of striatal degeneration, induced pluripotent stem cells were transplanted into the ipsilateral lateral ventricle 10 days after the quinolinic acid injection. At 8 weeks after transplantation, fluorodeoxyglucose-PET/CT scan and balance-beam test were performed to evaluate the functional recovery of experimental rats. In addition, immunofluorescence and protein array analysis were used to investigate the regulation of stimulated protein expression in the striatum.
Results: At 8 weeks after induced pluripotent stem cell transplantation, motor function was improved in comparison with the quinolinic acid-treated rats. High fluorodeoxyglucose accumulation in the injured striatum was also observed by PET/CT scans. In addition, immunofluorescence analysis demonstrated that implanted cells migrated from the lateral ventricle into the lesioned striatum and differentiated into striatal projection neurons. Array analysis showed a significant upregulation of GFR (Glial cell line-derived neurotrophic factor receptor) alpha-1, Adiponectin/Acrp30, basic-fibroblast growth factors, MIP-1 (Macrophage-inflammatory protein) alpha and leptin, as well as downregulation of cytokine-induced neutrophil chemoattractant-3 in striatum after transplantatation of induced pluripotent stem cells in comparison with the quinolinic acid -treated rats.
Conclusions: The findings in this work indicate that transplantation of induced pluripotent stem cells is a promising therapeutic candidate for HD.
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| http://dx.doi.org/10.1111/nan.12315 | DOI Listing |
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