AI Article Synopsis
- The study investigated the expression of Na,K-ATPase α and β subunit isoforms in colorectal cancer (CRC) cells and their liver metastases.
- The α1, α3, and β1 isoforms were predominantly found in tumor cells, with α1β1 having the highest Na(+) affinity and α3β1 the lowest, suggesting distinct roles in cancer cell function.
- A key finding was the expression of the α3β1 isozyme in liver metastatic CRC cells, indicating its potential as a new biomarker for these metastatic cells.
Article Abstract
The goal of this study was to define Na,K-ATPase α and β subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The α1, α3, and β1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. α1β1 and α3β1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na(+) affinity respectively and the highest K(+) affinity. Mesenchymal cell isozymes possess an intermediate Na(+) affinity and a low K(+) affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K(+) concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the α3β1 isozyme. Thus, the α3β1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731494 | PMC |
| http://dx.doi.org/10.3389/fphys.2016.00009 | DOI Listing |
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