Isolation and molecular identification of Naegleria fowleri from Nile river, Egypt.

J Egypt Public Health Assoc

Water Pollution Research Department, National Research Centre, Giza, Egypt.

Published: December 2015

Background: Members of the genus Naegleria are free-living amoebae distributed in various aquatic environments. Naegleria fowleri is the only species that can cause fatal primary amoebic meningoencephalitis in humans.

Materials And Methods: A total of 48 Nile water samples were collected from the water stream passing though Cairo. The samples were processed for the detection of Naegleria spp. using non-nutrient agar at 45°C. The isolates of Naegleria spp. were identified based on the morphologic criteria of trophozoite, flagellated and cyst stages. Molecular characterization of the isolates was performed using PCR.

Results: The obtained results showed that Naegleria spp. were found in 45.8% of Nile water samples by means of microscopic examination. Seasonally, the highest prevalence of Naegleria spp. was recorded in summer (66.7%). Moreover, the highest prevalence of N. fowleri was recorded in summer (25%).

Conclusion: The occurrence of heat-tolerant Naegleria spp., especially N. fowleri, in Nile water should be considered as a potential health threat.

Download full-text PDF

Source
http://dx.doi.org/10.1097/01.EPX.0000475937.97216.03DOI Listing

Publication Analysis

Top Keywords

naegleria spp
20
nile water
12
naegleria
8
naegleria fowleri
8
fowleri nile
8
water samples
8
highest prevalence
8
recorded summer
8
spp
5
isolation molecular
4

Similar Publications

Prevalence of in a Public Building Water Plumbing System During COVID-19 Lockdown.

Environ Health (Wash)

November 2023

Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, United States.

Article Synopsis
  • * The COVID-19 lockdown allowed researchers to gather data on how prolonged stagnation in a public building affected water quality and the population of free-living amoeba (FLA) species from June 2020 to May 2021.
  • * Findings indicate that long-term stagnation led to increased populations of specific FLA species, revealing important connections between water stagnation and microbial presence, which can guide future water quality management efforts.
View Article and Find Full Text PDF

A free-ranging, adult female two-toed sloth () was brought to a wildlife rescue center in Costa Rica with ocular and auricular myiasis and numerous skin lesions. After one month of unsuccessful systemic and topical antimicrobial treatment, the patient died. A postmortem examination was performed, and tissues were examined histologically, confirming disseminated amebic infection with intralesional trophozoites and cysts in the lungs, liver, eye, heart, spleen, and stomach.

View Article and Find Full Text PDF

This study aimed to investigate the presence of SARS-CoV-2, yeasts, and bacteria in isolated free-living amoeba (FLA) from COVID-19 patients. Nasopharyngeal swabs ( = 60) were obtained from COVID-19 patients. After cultivation, morphological characterization, and RNA/DNA extraction, the presence of selected microorganisms was investigated.

View Article and Find Full Text PDF

RAD51 recombinase and its paralogs: Orchestrating homologous recombination and unforeseen functions in protozoan parasites.

Exp Parasitol

December 2024

Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo #290, Col. Del Valle, CP 03100, Mexico City, Mexico. Electronic address:

The DNA of protozoan parasites is highly susceptible to damage, either induced by environmental agents or spontaneously generated during cellular metabolism through reactive oxygen species (ROS). Certain phases of the cell cycle, such as meiotic recombination, and external factors like ionizing radiation (IR), ultraviolet light (UV), or chemical genotoxic agents further increase this susceptibility. Among the various types of DNA damage, double-stranded breaks (DSBs) are the most critical, as they are challenging to repair and can result in genetic instability or cell death.

View Article and Find Full Text PDF
Article Synopsis
  • Free-living amoeba, though rarely harmful to humans, can cause severe central nervous system infections, as evidenced by two case reports of rapid illness progression.
  • The first case involved a 36-year-old male whose disease was linked to contaminated water, leading to fatal complications despite positive identification of the infection in cerebrospinal fluid (CSF).
  • The second case featured a 4-year-old boy with similar symptoms; despite treatment attempts, he also succumbed to cardiac arrest, highlighting the urgent need for awareness and early diagnosis of such parasitic infections.
View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!