Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation and cell surface hydrophobicity (CSH) are all virulence traits involved in the pathogenicity of Candida. Post-antifungal effect (PAFE) also have a bearing on pathogenicity and virulence of Candida. Candida dubliniensis is associated with oral and systemic candidosis, which can be managed with caspofungin. There is no published information on caspofungin-induced PAFE and its impact on adhesion traits of C. dubliniensis isolates. Thus, the purpose of this investigation was to determine the in vitro duration of PAFE on 20 C. dubliniensis isolates following transient exposure to caspofungin. Furthermore the impacts of caspofungin-induced PAFE on adhesion to BEC and DAS, GT formation and CSH of these isolates were also determined. After establishing the minimum inhibitory concentration (MIC) of caspofungin, C. dubliniensis isolates were exposed to sub-lethal concentrations (×3 MIC) of caspofungin for 1 hr. Thereafter the duration of PAFE, adhesion to BEC and DAS, GT formation and CSH were determined by previously described in-vitro assays. MIC (μg/mL) of C. dubliniensis isolates to caspofungin ranged from 0.004 to 0.19. Caspofungin-induced mean PAFE on C. dubliniensis isolates was 2.17 hr. Exposure to caspofungin suppressed the ability of C. dubliniensis isolates to adhere to BEC and DAS, form GT and CSH by 69.97%, 71.95%, 90.06% and 32.29% (P < 0.001 for all), respectively. Thus, transient exposure of C. dubliniensis isolates to caspofungin produces an antifungal effect not only by suppressing its growth but also by altering its adhesion traits.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/1348-0421.12362 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Co-culture of Helicobacter pylori with oral microorganisms in human saliva.
Clin Oral Investig
January 2025
Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center- University of Freiburg, Faculty of Medicine, University of Freiburg, University of Freiburg, Freiburg, Germany.
Objective: Helicobacter pylori is known for colonizing the gastric mucosa and instigating severe upper gastrointestinal diseases such as gastritis, gastroduodenal ulcers, and gastric cancer. To date, there is no data available on the oral cavity as transmission site, whether H. pylori can survive in the oral cavity or in human saliva.
View Article and Find Full Text PDFAnidulafungin is a useful surrogate marker for predicting susceptibility to rezafungin among five species using CLSI methods and interpretive criteria.
J Clin Microbiol
January 2025
Element Iowa City (JMI Laboratories), North Liberty, Iowa, USA.
This study addresses the use of other echinocandins as surrogate markers to predict the susceptibility of rezafungin against the six most common spp. The Clinical Laboratory Standards Institute (CLSI) reference broth microdilution method was performed to test 5,720 clinical isolates of six different species. Species-specific interpretative criteria by CLSI breakpoints or epidemiological cutoff values were applied.
View Article and Find Full Text PDFPhenotypic and genotypic characterization of species from the oral cavity of healthy individuals in Lublin province, Poland.
J Oral Microbiol
December 2024
Innovation Research Centre, John Paul II University in Biała Podlaska, Biala Podlaska, Poland.
Article Synopsis
- The study investigates the types of fungi, specifically Candida species, found in the mouths of healthy individuals and their genetic diversity.
- Out of 500 participants, different species of Candida were identified, with 130 individuals carrying them, among which C. albicans was the most prevalent.
- Results reveal a complex relationship between the genotypes and phenotypes of Candida, suggesting that using both microbiological and molecular techniques can improve the identification of these fungi.
Distribution of Candida species isolated from people living with human immunodeficiency virus with oropharyngeal and oral candidiasis in Africa in the era of universal test and treat policy: a systematic review and meta-analysis.
Trop Med Health
November 2024
Department of Medical Microbiology, School of Biomedical Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda.
Article Synopsis
- The study examines the prevalence of Candida species causing oropharyngeal and oral candidiasis among individuals with HIV in Africa, in light of improved treatments like antiretroviral therapy and the universal test and treat (UTT) policy.
- It analyzed data from 14 studies involving over 4,200 participants, finding that a significant majority of the Candida species isolated were C. albicans, while non-albicans species like C. glabrata and C. tropicalis were also present.
- The research indicates a decrease in the prevalence of oropharyngeal candidiasis since the introduction of UTT, with a pooled prevalence of 48%, suggesting effective interventions, although certain challenges remain.
The In Vitro Activity of Rezafungin Against Uncommon Species of Candida.
Mycoses
November 2024
Element Iowa City (JMI Laboratories), North Liberty, Iowa, USA.
Background: Invasive candidiasis (IC) is increasing due to the rising numbers of immunocompromised patients. Increasing azole resistance rates and daily dosing required for most echinocandins have complicated its treatment. The approval of rezafungin has provided an option for weekly echinocandin treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!