Species composition and concentration of rumen ciliate protozoa were investigated in the rumen contents of 14 domestic sheep and 1 goat living in Bishkek, Kyrgyzstan. This is the first report on rumen ciliates from ruminants living in Kyrgyzstan. In sheep 12 genera, 28 species and 12 morphotypes were detected, whereas in goat 8 genera, 12 species and 4 morphotypes were detected. The density of ciliates in sheep was (28.1 ± 20.0) × 10(4) cells mL(-1) and in goat was 37.0 × 10(4) cells mL(-1). Dasytricha ruminantium, Isotricha prostoma, Entodinium simulans and Ophryoscolex caudatus were major species (100%) in sheep, and for the first time, Diplodinium rangiferi was detected in a domestic goat.
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http://dx.doi.org/10.1093/femsle/fnw028 | DOI Listing |
Metagenomics, particularly genome-resolved metagenomics, has significantly deepened our understanding of microbes, illuminating their taxonomic and functional diversity and roles in ecology, physiology, and evolution. However, eukaryotic populations within various microbiomes, including those in the mammalian gastrointestinal (GI) tract, remain relatively underexplored in metagenomic studies due to the lack of comprehensive reference genome databases and robust bioinformatics tools. The GI tract of ruminants, particularly the rumen, contains a high eukaryotic biomass although a relatively low diversity of ciliates and fungi, which significantly impacts feed digestion, methane emissions, and rumen microbial ecology.
View Article and Find Full Text PDFJ Nutr
December 2024
National Center for International Research on Animal Gut Nutrition, Jingsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Nanjing Agricultural University, Nanjing, 210095 China; College of Animal Science & Technology, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address:
Background: Rumen methane emissions (RME) significantly contribute to global greenhouse gas emissions, underscoring the essentials to identify effective inhibitors for RME mitigation. Despite various inhibitors shown potential in reducing RME by modulating rumen microbes, their impacts include considerable variations and inconsistency.
Objective: We aimed to quantitively assess the impacts of various methane inhibitors on RME, rumen microbial abundance and fermentation in ruminants.
Eur J Protistol
October 2024
Laboratório de Diversidade Genética, Departamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
Rumen ciliates constitute a highly diverse group within the subclass Trichostomatia. They are known for their symbiotic relationship with ruminant hosts and their pivotal role in digestive metabolism. This review presents a historical analysis of research on rumen ciliate metabolism, since the earliest studies, based on in vitro and in situ experiments, as well as molecular studies, initially relying on Sanger sequencing, and more recently, next-generation sequencing techniques.
View Article and Find Full Text PDFMethodsX
December 2024
Department of Pathogenic Biology, School of Medicine, Jianghan University, Wuhan, China.
The rumen ciliates are a diverse group of protozoa residing in the rumen of ruminant animals. They are primarily found in the orders Entodiniomorphida and Vestibuliferida, playing crucial roles in the digestion and breakdown of feed within the host's rumen, closely intertwined with the host's nutrient absorption. monocultures of representatives of rumen ciliates are important to better study them.
View Article and Find Full Text PDFEnviron Microbiol Rep
August 2024
Department of Ruminant Science, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel.
Ciliate protozoa are an integral part of the rumen microbial community involved in a variety of metabolic processes. These processes are thought to be in part the outcome of interactions with their associated prokaryotic community. For example, methane production is enhanced through interspecies hydrogen transfer between protozoa and archaea.
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