AI Article Synopsis
- Photosynthetic bradyrhizobia can induce nitrogen-fixing nodules on certain Aeschynomene species through a Nod-factor independent method, using a unique sugar in their LPS O-antigen called bradyrhizose, which doesn't trigger immune responses.
- Researchers studied mutants of the ORS285 strain to understand the role of the O-antigen by screening over 10,000 mutants and identifying five specific mutations in genes critical for O-antigen synthesis.
- Despite the mutants lacking the O-antigen, biochemical analysis showed that they still maintained their symbiotic capabilities with Aeschynomene, indicating that the O-antigen is not essential for this symbiotic relationship.
Article Abstract
The photosynthetic bradyrhizobia are able to use a Nod-factor independent process to induce nitrogen-fixing nodules on some semi-aquatic Aeschynomene species. These bacteria display a unique LPS O-antigen composed of a new sugar, the bradyrhizose that is regarded as a key symbiotic factor due to its non-immunogenic character. In this study, to check this hypothesis, we isolated mutants affected in the O-antigen synthesis by screening a transposon mutant library of the ORS285 strain for clones altered in colony morphology. Over the 10,000 mutants screened, five were selected and found to be mutated in two genes, rfaL, encoding for a putative O-antigen ligase and gdh encoding for a putative dTDP-glucose 4,6-dehydratase. Biochemical analysis confirmed that the LPS of these mutants completely lack the O-antigen region. However, no effect of the mutations could be detected on the symbiotic properties of the mutants indicating that the O-antigen region of photosynthetic Bradyrhizobium strains is not required for the establishment of symbiosis with Aeschynomene.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743980 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148884 | PLOS |
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