A nanochannel with a shutter at one end was built by DNA nanotechnology. Using DNA hybridization the shutter could be opened or closed, influencing the transport of materials through the channel. This process was visualized by an enzyme cascade reaction occurring in the structure.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1039/c5nr08206d | DOI Listing |
ACS Meas Sci Au
December 2024
Department of Bioengineering and Nano-Bioengineering, Research Center for Bio Materials and Process Development, Incheon National University, Incheon 22012, Republic of Korea.
Thermal cycling-based quantitative polymerase chain reaction (qPCR) represents the gold standard method for accurate and sensitive nucleic acid quantification in laboratory settings. However, its reliance on costly thermal cyclers limits the implementation of this technique for rapid point-of-care (POC) diagnostics. To address this, isothermal amplification techniques such as rolling circle amplification (RCA) have been developed, offering a simpler alternative that can operate without the need for sophisticated instrumentation.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Key Laboratory of the Evaluation and Monitoring of Southwest Land Resources (Ministry of Education), Sichuan Normal University, Chengdu, 610066, China; College of Chemistry and Materials Science, Sichuan Normal University, Chengdu, 610066, China. Electronic address:
Background: Pesticide residues can cause chronic toxicity to the human body and lead to a series of diseases that damage the liver. Therefore, developing a highly sensitive, selective, and low-cost pesticide residues detection method is of great significance for protecting human health and safety. Nowadays, commonly used methods for pesticide residue detection include gas chromatography, high-performance liquid chromatography, and fluorescence sensing.
View Article and Find Full Text PDFJ Am Chem Soc
December 2024
Guangdong Provincial Key Laboratory of Digestive Cancer Research, Precision Medicine Center, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518107, China.
Cellular context profiling of modification effector proteins is critical for an in-depth understanding of their biological roles in RNA -methyladenosine (mA) modification regulation and function. However, challenges still remain due to the high context complexities, which call for a versatile toolbox for accurate live-cell monitoring of effectors. Here, we propose a demethylation-switchable aptamer sensor engineered with a site-specific mA (DSA-mA) for lag-free monitoring of the mA demethylase FTO activity in living cells.
View Article and Find Full Text PDFChem Sci
December 2024
Marshall Laboratory of Biomedical Engineering, Precision Medicine and Health Research Institute, Research Center for Biosensor and Nanotheranostic, Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University Shenzhen 518060 China
Aberrant microRNA (miRNA) expression is associated with various types of carcinogenesis, making miRNA a promising candidate for diagnostic and therapeutic biomarkers. However, miRNA diagnostics remains a significant challenge owing to the various biological barriers. Herein, we report a novel miRNA imaging probe consisting of PEG-polylysine-PNIPAM polymer matrix-modified small FeO (PAA-FeO-DNA@PPP) nanoparticles with an improved circulatory half-life, efficient tissue permeability, and enhanced tumor accumulation, for miRNA magnetic resonance imaging (MRI).
View Article and Find Full Text PDFACS Chem Neurosci
December 2024
Physical and Materials Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra 411008, India.
The aberrant aggregation of TAR DNA-binding protein 43 kDa (TDP-43) in cells leads to the pathogenesis of multiple fatal neurodegenerative diseases. Decoding the proposed initial transition between its functional dimeric and aggregation-prone monomeric states can potentially design a viable therapeutic strategy, which is presently limited by the lack of structural detail of the full-length TDP-43. To achieve a complete understanding of such a delicate phase space, we employed a multiscale simulation approach that unearths numerous crucial features, broadly summarized in two categories: (1) state-independent features that involve inherent chain collapsibility, rugged polymorphic landscape dictated by the terminal domains, high β-sheet propensity, structural integrity preserved by backbone-based intrachain hydrogen bonds and electrostatic forces, the prominence of the C-terminal domain in the intrachain cross-domain interfaces, and equal participation of hydrophobic and hydrophilic (charged and polar) residues in cross-domain interfaces; and (2) dimerization-modulated characteristics that encompass slower collapsing dynamics, restricted polymorphic landscape, the dominance of side chains in interchain hydrogen bonds, the appearance of the N-terminal domain in the dimer interface, and the prominence of hydrophilic (specifically polar) residues in interchain homo- and cross-domain interfaces.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!