AI Article Synopsis
- Mesenchymal stem cells (MSCs) show promise for regenerative medicine, but their clinical use requires consistent preparation methods to prevent unintended differentiation due to various environmental factors.
- This study examined how different levels of hydrodynamic loading affect the differentiation of MSCs into bone and cartilage lineages when supplemented with factors like TGF-β3 and BMP-2 under static and dynamic conditions.
- Results indicated that while hydrodynamic loading alone had limited impact, it enhanced gene expression for bone and cartilage formation when combined with the inductive factors, particularly at high shear rates; however, care must be taken to avoid unwanted osteogenic differentiation in chondrogenic cultures.
Article Abstract
Background: Mesenchymal stem cells (MSCs) are viewed as a having significant potential for tissue engineering and regenerative medicine therapies. Clinical implementation of MSCs, however, demands that their preparation be stable and reproducible. Given that environmental and bioprocessing parameters such as substrate stiffness, seeding densities, culture medium composition, and mechanical loading can result in undirected differentiation of the MSC population, the objective of this study was to systematically investigate how hydrodynamic loading influences the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) towards the osteochondral lineages both in the presence and absence of exogenous, inductive factors.
Methods: Expanded bovine MSCs were suspended in 2.5 % agarose, cast in a custom mold, and placed into either static or one of two dynamic culture environments consisting of "high" and "low" magnitude shear conditions. Constructs were supplemented with varying concentrations (0, 1, 10, 100 ng/mL) of either TGF-β3 or BMP-2 throughout cultivation with tissue samples being collected following each week of culture.
Results: In the absence of exogenous supplementation, hydrodynamic loading had little effect on cell phenotype at either magnitude of stimulation. When cultures were supplemented with BMP-2 and TGF-β3, MSCs gene expression progressed towards the osteogenic and chondrogenic pathways, respectively. This progression was enhanced by the presence of hydrodynamic loading, particularly under high shear conditions, but may point the chondrogenic cultures down a hypertrophic path toward osteogenesis reminiscent of endochondral ossification if TGF-β3 supplementation is insufficient.
Conclusions: Moving forward, these results suggest bioprocessing conditions which minimize exposure of chondrogenic cultures to fluid shear stress to avoid undesirable differentiation of the MSC population.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736240 | PMC |
| http://dx.doi.org/10.1186/s12896-016-0240-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Boosting Therapeutic Effect of Turmeric, Coffee, and Chili Extracts Through Experimental Design and Encapsulation as Nanostructured Lipid Carriers for Novel Heath Supplements.
Plants (Basel)
January 2025
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
This study investigates the potential synergistic effects of extracts from (turmeric), (Arabica coffee beans), and (chili peppers) in reducing oxidative stress and inflammation, which are associated with metabolic disorders such as obesity, diabetes, and cardiovascular diseases. Using a systematic design of experiment (DoE) optimization approach, an optimal extract ratio of 1:3:4 (turmeric: coffee: chili) was identified. The efficacy of the extract combination was assessed through various antioxidant assays, inhibition of inflammation-related gene expression, and safety testing via the 3-(4,5-dimethylthazolk-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
View Article and Find Full Text PDFInvestigation of Chip Morphology in Elliptical Vibration Micro-Turning of Silk Fibroin.
Micromachines (Basel)
January 2025
Centre for Precision Manufacturing, DMEM, University of Strathclyde, Glasgow G1 1XJ, UK.
Silk fibroin, known for its biocompatibility and biodegradability, holds significant promise for biomedical applications, particularly in drug delivery systems. The precise fabrication of silk fibroin particles, specifically those ranging from tens of nanometres to hundreds of microns, is critical for these uses. This study introduces elliptical vibration micro-turning as a method for producing silk fibroin particles in the form of cutting chips to serve as carriers for drug delivery systems.
View Article and Find Full Text PDFContribution of Phosphorylation Modification to Stability and Antibacterial Activity of Egg White Protein Nanogels Loaded with Cinnamon Bark Essential Oil.
Gels
December 2024
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.
This study evaluated the potential usage of phosphorylated egg white protein (P-EWP) nanogels fabricated via microwave-induced phosphorylation modification and gel process and further ultrasonic nanometrization as novel delivery systems for cinnamon bark essential oil (CBEO). Compared to EWP-CBEO nanogels without chemical phosphorylation, the obtained P-EWP-CBEO nanogels have shown smaller average hydrodynamic diameter (133.6 nm), relatively uniform size distribution (polydispersity index around 0.
View Article and Find Full Text PDFDynamics search of highly magnetized blood laden with copper-gold-titania nanoparticles in a ciliary artery with catheterization and entropy.
Electromagn Biol Med
January 2025
Department of Mathematics, University of Gour Banga, Malda, India.
Article Synopsis
- Biomagnetic fluid dynamics (BFD) focuses on the behavior of bio-fluids, like blood, impacted by magnetic fields, which is important for medical applications such as targeted medication delivery and tumor treatment.
- This study examines blood flow dynamics using trihybrid nanoparticles in a catheterized artery, factoring in various electromagnetic influences and propulsion mechanisms.
- Key findings include that increasing Hall and ion-slip parameters boosts blood velocity, modifies entropy generation, and shows that modified hybrid nano-blood forms smaller, more manageable clumps compared to pure blood, with longer cilia enhancing recovery of these clumps.
Sustainable management of riverine NO emission baselines.
Natl Sci Rev
February 2025
Key Laboratory of Water and Sediment Sciences, Ministry of Education, Department of Environmental Engineering, Peking University, Beijing 100871, China.
The riverine NO fluxes are assumed to linearly increase with nitrate loading. However, this linear relationship with a uniform EF is poorly constrained, which impedes the NO estimation and mitigation. Our meta-analysis discovered a universal NO emission baseline (EF = k/[NO ], k = 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!