AI Article Synopsis
- The study examines the differences in CD4+ T-cell subsets between dyslipidemic patients and healthy subjects, focusing on the regulatory T cells (CD4+ CD25(high)FoxP3+) and their response to atorvastatin treatment.
- The results indicate that while CD4+ CD25(high)FoxP3+ cells were more prevalent in patients, their frequency remained unchanged during atorvastatin therapy, and other cell functions were not significantly modified in vitro.
- Findings suggested a correlation between CD4+ CD25(high)FoxP3+ cells and cholesterol levels, highlighting their potential role in atherosclerosis development, although statin treatment did not achieve the expected immuno-modulation in these T-cell subsets.
Article Abstract
Background: The CD4+ T-lymphocytes and their subtype CD4 + CD25(high)FoxP3+ regulatory T cells are receiving growing interest as major regulators of atherogenesis. We sought to investigate 1) whether the CD4 + cell subsets were expressed differently in dyslipidemic patients (Pts) and healthy subjects (HS) and 2) whether atorvastatin treatment could be associated in-vivo and in-vitro with cell changes in expression and functional response.
Methods: CD4+ subsets frequency (CD4 + CD25(high)FoxP3+, CD4 + CD25-FoxP3+) and mRNA expression for FoxP3, IL-10 and TGF-β were evaluated in 30 consecutive Pts at baseline and after a 3-month atorvastatin therapy, and in 17 HS.
Results: The % of CD4 + cells did not differ between HS and Pts. The % of CD4 + CD25(high)FoxP3+ was higher in Pts than HS and did not change during treatment. The CD4 + CD25-FoxP3+ cells were similar between the two groups and were lower in Pts at visit 2. Cytokine expression and FoxP3 did not differ in HS and Pts and no substantial change was observed during treatment. At visit 1, CD4 + CD25(high)FoxP3+ cells were significantly correlated with both total-cholesterol (r = 0.570, P = 0.0002), LDL-cholesterol (r = 0.715, P = 0.0001), Apolipoprotein B (r = 0.590, P = 0.0001). In-vitro atorvastatin (up to 5 μM) failed to induce any significant modulation of cell functions.
Conclusion: CD4 + CD25(high)FoxP3+ regulatory cells seem to be over-stimulated in the early pre-clinical phase of atherosclerosis and a relationship exists between their frequency and circulating lipids. A potential immuno-modulation by statin treatment is not achieved through a normalization in peripheral CD4 + cell subsets.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731979 | PMC |
| http://dx.doi.org/10.1186/s12872-016-0201-y | DOI Listing |
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