AI Article Synopsis
- The chapter covers the process of producing recombinant proteins using the baculovirus expression vector system (BEVS) in large bioreactors, detailing the stages from host cell growth to product recovery.
- It outlines methods for virus amplification, bioreactor setup and operation, and the steps involved in infection and harvesting the final product.
- The text also touches on advanced monitoring and control techniques that are crucial for improving the success of the production process.
Article Abstract
The production of recombinant proteins using the baculovirus expression vector system (BEVS) in large-scale agitated bioreactors is discussed in this chapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-3043-2_11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Facile and enlargeable preparation of piezocatalytic CaCO for efficient degradation of organic dyes.
Environ Res
December 2024
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical & Material Engineering Jiangnan University, Wuxi, 214122, PR China. Electronic address:
Piezoelectric catalysis has emerged as a promising green technology for implementing pollutant degradation and HO production. The use of environment friendly and abundantly available piezocatalysts is critical for practical applications. This work presents the preparation of various morphological CaCO via a simple precipitation method in the absence or presence of different templates.
View Article and Find Full Text PDFPericardial Fenestration With Monitored Anesthesia Care Using Diluted Remifentanil: A Series of 10 Cases.
Cureus
November 2024
Department of Anaesthesiology, Seirei Hamamatsu General Hospital, Hamamatsu, JPN.
Introduction Monitored anesthesia care (MAC) is an anesthesia management method in which anesthesiologists use the minimum necessary intravenous anesthetics to achieve sedation and pain relief while maintaining spontaneous breathing. The challenge for the anesthesiologist is to find the correct balance between inhibiting the patient's stress response to invasive treatment, maintaining intraoperative hemodynamic stability, and doing so as quickly as possible. We hypothesized that diluting remifentanil (D-remi) to 10 μg/mL and increasing the rate of administration would increase the responsiveness of the syringe pump and allow for better control of anesthetic depth.
View Article and Find Full Text PDFAnalgosedation in Pediatric Emergency Care: A Comprehensive Scoping Review.
Pharmaceuticals (Basel)
November 2024
Pediatric Clinic, Department of Medicine and Surgery, University Hospital of Parma, 43126 Parma, Italy.
Effective management of pain and anxiety in pediatric emergency room is crucial for ensuring both the physical and emotional well-being of young patients. Analgosedation, a combination of analgesia and sedation, is commonly used to facilitate various procedures in children. However, selecting the optimal agent and administration route remains challenging due to the unique pharmacological profiles and side effects of available drugs.
View Article and Find Full Text PDFLarge-Scale Transient Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the Production of the Trimeric SARS-CoV-2 Spike Protein.
Methods Mol Biol
October 2024
Protein Production and Structure Core Facility (PTPSP), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
The production and purification of the secreted ectodomain of SARS-CoV-2 spike protein (S protein) were performed by transiently transfecting suspension-adapted Chinese hamster ovary cells (ExpiCHO). The method involved the separate addition of plasmid DNA expressing the S protein and polyethyleneimine to a suspension culture at a density of 5 × 10 cells/mL; and the subsequent addition of dimethyl sulfoxide at 2% (v/v). The transfected ExpiCHO cells were cultivated at 31 °C with agitation by orbital shaking under 5% CO.
View Article and Find Full Text PDFEvaluation of large-scale poultry manure-derived biochar for efficient cadmium removal in zinc smelter wastewater.
J Environ Manage
November 2024
Institute of Agricultural Environmental Science, Hankyong National University, Anseong, 17579, Republic of Korea; Department of Bioresources and Rural System Engineering, Hankyong National University, Anseong, 17579, Republic of Korea. Electronic address:
Cadmium (Cd), a carcinogen, is released from industrial activities like metal refineries and battery runoff, with significant contamination reported near zinc smelters in Korea. This study addresses the issue using an efficient, economical adsorption process with waste-derived biochar-based adsorbents known for high Cd removal. Poultry manure (PM), typically used as fertilizer, can lead to environmental pollution if mismanaged; therefore, it was pyrolyzed to produce biochar.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!