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Circular RNAs in extracellular vesicles (EV-circRNAs) are gaining recognition as potential biomarkers for the diagnosis of gastric cancer (GC). Most current research is focused on identifying new biomarkers and their functional significance in disease regulation. However, the practical application of EV-circRNAs in the early diagnosis of GC is yet to be thoroughly explored due to the low accuracy of EV-circRNAs analysis.
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State Key Laboratory of Quality Research in Chinese Medicines & School of Pharmacy, Faculty of Medicine, Macau University of Science and Technology, Macau 999078, China. Electronic address:
Although electrochemical biosensors have been developed to detect multiple microRNAs (miRNAs) simultaneously through loading different capture probes, high surface-induced perturbation and competition among probes have reduced the detection sensitivity. To address these challenges, a trefoil DNA capture probe (TDCP) was designed for microRNA-21 (miR-21) and microRNA-16 (miR-16) detection simultaneously. The TDCP exhibits a stable structure, low spatial resistance, and integral rigidity, which decreases high surface-induced perturbations and competition to improve the accessibility of the target miRNA.
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