Near-freezing effects on the proteome of industrial yeast strains of Saccharomyces cerevisiae.

J Biotechnol

Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Av. Agustín Escardino, 7, 46980 Paterna, Valencia, Spain. Electronic address:

Published: March 2016

At near-freezing temperatures (0-4°C), the growth of the yeast Saccharomyces cerevisiae stops or is severely limited, and viability decreases. Under these conditions, yeast cells trigger a biochemical response, in which trehalose and glycerol accumulate and protect them against severe cold and freeze injury. However, the mechanisms that allow yeast cells to sustain this response have been not clarified. The effects of severe cold on the proteome of S. cerevisiae have been not investigated and its importance in providing cell survival at near-freezing temperatures and upon freezing remains unknown. Here, we have compared the protein profile of two industrial baker's yeast strains at 30°C and 4°C. Overall, a total of 16 proteins involved in energy-metabolism, translation and redox homeostasis were identified as showing increased abundance at 4°C. The predominant presence of glycolytic proteins among those upregulated at 4°C, likely represents a mechanism to maintain a constant supply of ATP for the synthesis of glycerol and other protective molecules. Accumulation of these molecules is by far the most important component in enhancing viability of baker's yeast strains upon freezing. Overexpression of genes encoding certain proteins associated with translation or redox homeostasis provided specifically protection against extreme cold damage, underlying the importance of these functions in the near-freezing response.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jbiotec.2016.01.029DOI Listing

Publication Analysis

Top Keywords

yeast strains
12
saccharomyces cerevisiae
8
near-freezing temperatures
8
yeast cells
8
severe cold
8
baker's yeast
8
translation redox
8
redox homeostasis
8
yeast
6
near-freezing
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!