AI Article Synopsis

  • Trematodes are emerging parasites in tropical areas, specifically linked to ocular granulomas in children who bathe in South Indian water bodies.
  • DNA from surgically removed granulomas and trematode larvae was analyzed using PCR techniques to identify the specific trematode responsible.
  • The study confirmed that Procerovum varium, a type of trematode, is the cause of the ocular infections, highlighting the role of snail vectors as environmental risks.

Article Abstract

Trematodes are recognized as a group of emerging parasites in tropical countries. We identified a trematode as a cause of ocular granulomas that developed in children who bathed in ponds or rivers in South India. DNA was isolated from patients' surgically excised granulomas and from the trematode cercariae (larvae) released by the snail Melanoides tuberculata in water in which the children bathed. Real-time and conventional PCRs were performed that targeted ribosomal DNA regions spanning the internal transcribed spacer 2 and 28S sequences of this trematode. The PCR-amplified products were subjected to bidirectional sequencing. Analysis of sequences for the granuloma samples and the trematode cercariae showed maximum sequence similarity with Procerovum varium (family Heterophyidae). Our results confirmed the etiology of the ocular infection, implicating snail vectors as environmental risk factors for ocular parasitosis.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734527PMC
http://dx.doi.org/10.3201/eid2202.150051DOI Listing

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