Aims: The study aimed to perform a systematic investigation of the effects of quercetin on biofilm formation and virulence factors in Pseudomonas aeruginosa.
Methods And Results: The Ps. aeruginosa strain PAO1 was selected as the test strain. The results indicated that quercetin did not impact the growth of PAO1 as determined by MIC and growth curve analysis. However, this compound significantly inhibited (P < 0·05) biofilm formation and production of virulence factors including pyocyanin, protease and elastase at a lower concentration than those for most previously reported plant extracts and substances. Considering the central role of quorum sensing (QS) in the regulation of biofilm and virulence factor, we further detected the transcriptional changes associated with QS and found that the expression levels of lasI, lasR, rhlI and rhlR were significantly reduced (P < 0·05) by 34, 68, 57 and 50%, respectively, in response to 16 μg ml(-1) quercetin.
Conclusions: This study indicated that quercetin is an effective inhibitor of biofilm formation and virulence factors in Ps. aeruginosa.
Significance And Impact Of The Study: This is the first study to demonstrate that quercetin is an effective inhibitor of QS, biofilm formation and virulence factors in Ps. aeruginosa. Furthermore, quercetin might have potential in fighting biofilm-related infections.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jam.13073 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative phenotypic and proteomic analysis of colistin-exposed .
Germs
September 2024
PhD, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam, and Research Center for Infectious Diseases, International University, Vietnam National University, Ho Chi Minh City 700000, Vietnam.
Introduction: The emergence of colistin resistance threatens the treatment of infections.
Methods: In this study, in vitro development of colistin resistance was investigated using comparative phenotypic and proteomic analysis of ATCC 9027, its 14-day colistin sub-MIC exposed strain (Col-E1), and 10-day antibiotic-free cultured Col-E1 strain (Col-E2). Antibiotic susceptibility, morphology, virulence factors, and proteomic changes were assessed using disc-diffusion, agar-based, spectrophotometry, SEM, and iTRAQ-LC-MS/MS methods.
Nematode-bacteria interactions in bovine parasitic otitis.
Rev Bras Parasitol Vet
January 2025
Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro - UERJ, Rio de Janeiro, RJ, Brasil.
Bovine parasitic otitis poses challenges in diagnosis, treatment and involves various agents, such as bacteria, fungi, mites, and nematodes. This study focused on the nematodes and bacteria isolated from the auditory canals of dairy cattle. A total of twenty samples were collected from dairy cattle in two states of Brazil.
View Article and Find Full Text PDFMicrobial boundaries in peri-implantitis: a review of pathogen-related advances.
Folia Med (Plovdiv)
December 2024
University of Baghdad, Baghdad, Iraq.
In the field of implantology, peri-implantitis is still a common complication of implant failure. Similar to periodontal disease, this kind of pathological condition is characterized by inflammation of the tissues surrounding dental implants or fillings. The sources of infection have been shown to be chronic periodontitis and poor maintenance of the communion.
View Article and Find Full Text PDFQuorum quenching nanoparticles against wound pathogens - A scoping review.
Med J Malaysia
January 2025
Nanobiomedicine lab, Centre for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
Introduction: Quorum sensing (QS) enables bacteria to coordinate colony-wide activities, including those associated with infections. Quorum quenching (QQ) inhibits QS and is a promising method for controlling bacterial infections. Several In vitro experiments have been conducted to identify nanoparticles (NPs) as potential quorum quenching inhibitors.
View Article and Find Full Text PDFNatural products in the treatment of diabetic foot infection.
Eur J Med Res
January 2025
Research Center for Molecular Medicine, Institute of Cancer, Avicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
Diabetic foot infections (DFIs) are a significant complication in diabetes mellitus, leading to increased morbidity, hospitalizations, and healthcare burdens. The growing prevalence of antibiotic-resistant pathogens has reduced the efficacy of conventional treatments, highlighting the need for alternative therapeutic strategies. Natural products, known for their antimicrobial, anti-inflammatory, and wound-healing properties, have garnered attention as potential treatments for DFIs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!