Characterization of Soybean Storage and Allergen Proteins Affected by Environmental and Genetic Factors.

J Agric Food Chem

Animal Biosciences and Biotechnology Laboratory, USDA-ARS , Beltsville, Maryland 20705, United States.

Published: February 2016

AI Article Synopsis

  • The study examined how genetics and environmental factors affect protein composition in soybeans, focusing on the role of genotype (G), environment (E), and their interaction (G×E).
  • Three sets of nine soybean genotypes were tested across different locations (Maryland, South Carolina, South Dakota) with varying planting dates.
  • Analysis of protein expression revealed that environmental differences had a more significant impact on soybean protein variability than genetic differences or the interaction between genotype and environment.

Article Abstract

There is limited information on the influence of genetic and environmental variability on soybean protein composition. This study aimed to determine the role of genotype (G), environments (E), and the interrelationship of genotype and environment (G×E) on soybean seed protein. Three sets of nine soybean genotypes were grown in replicated trials at Maryland, South Carolina, and South Dakota. At each location, the nine genotypes were grown with two planting/sowing dates. We applied two-dimensional gel electrophoresis and mass spectrometry to study the variability of soybean storage and allergen proteins. Statistical analysis of 47 storage and 8 allergen proteins, in terms of differentially expressed protein spots significant at the p<0.005 level, was performed. We found more spots that showed statistically significant differences in expression among E compared to G and G×E interaction.

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http://dx.doi.org/10.1021/acs.jafc.5b05172DOI Listing

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