AI Article Synopsis
- The study focuses on creating a new 3D matrix for bone tissue engineering, incorporating collagen, hydroxyapatite, β-cyclodextrin, and usnic acid with antimicrobial properties.
- Various characterization techniques, including SEM, FT-IRM, TEM, and XRD, were employed to assess the matrix's structure and properties.
- The matrix demonstrated biocompatibility with MG-63 osteoblast-like cells and showed significant antimicrobial effects, particularly against Staphylococcus aureus, suggesting it as a promising option for regenerative medicine.
Article Abstract
The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), β-cyclodextrin (β-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273771 | PMC |
| http://dx.doi.org/10.3390/molecules21010115 | DOI Listing |
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