Candida guilliermondii Wickerham was found to be superior to the other yeasts tested for growth yields and riboflavin production when cultivated on different carbohydrate and hydrocarbon-containing media. Among the refined petroleum fractions and cheap hydrocarbons tested, solar was selected as a carbon source best suited for the fermentation process. The highest growth yield and riboflavin output (10.64 mg/100 ml) by C. guilliermondii Wickerham were achieved by placing aliquots of 20 ml medium (pH 6.0) in 100 ml Erlenmeyer flasks and incubating the inoculated medium at 30 degrees C for 14 days in the dark.
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Rev Cubana Med Trop
April 2013
Departamento de Investigaciones de Medios de Cultivo, Centro Nacional de Biopreparados (BioCen), Ciudad de La Habana, Cuba.
Introduction: Candida species are responsible for the most common opportunistic mycotic infections. Rapid and accurate identification of yeast has become relevant not only for the effective management of infections, but also for the prevention of drug resistance. Up to now, yeast identification procedures are based on a variety of tests, including their ability to use sugars.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2000
Electrophoretic karyotypes of 15 type strains of the taxa in the Candida guilliermondii complex including Candida fukuyamaensis Nakase et al. and Candida xestobii Yarrow et S. A.
View Article and Find Full Text PDFZentralbl Mikrobiol
July 1992
Botany Department, Faculty of Science, Alexandria University, Egypt.
The production of riboflavin (vitamin B2) by Candida guilliermondii Wickerham cultivated on solar-containing medium was stimulated in the presence of corn oil (0.1 g%), arginine, phenylalanine (1 m mole/l) or CoSO4.7H2O (1000 micrograms/l).
View Article and Find Full Text PDFMicrobiologia
June 1989
Botany Department, Faculty of Science, Alexandria University, Egypt.
Candida guilliermondii Wickerham was found to be superior to the other yeasts tested for growth yields and riboflavin production when cultivated on different carbohydrate and hydrocarbon-containing media. Among the refined petroleum fractions and cheap hydrocarbons tested, solar was selected as a carbon source best suited for the fermentation process. The highest growth yield and riboflavin output (10.
View Article and Find Full Text PDFAlkaline nucleotide pyrophosphatase was isolated from the Pichia guilliermondii Wickerham ATCC 9058 cell-free extracts. The enzyme was 740-fold purified by saturation of ammonium sulphate, gel-chromatography on Sephadex G-150 and ion-exchange chromatography on DEAE-cellulose. Nucleotide pyrophosphatase is the most active at pH 8.
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