During a nine-month period in 1986, we observed five ornithine decarboxylase-negative isolates of Serratia marcescens from four different patients. All isolates were identical in more than 50 biochemical parameters. Four isolates from three hospitalized patients were essentially identical in their susceptibility patterns to 12 antimicrobial agents. Analysis of 35-S-methionine labeled whole cell proteins by SDS-PAGE with the AMBIS system (Automated Microbiology System Inc., San Diego, CA) suggested that only three of these isolates were identical while the fourth hospital strain was more closely related to the isolate of a patient with no contact to the hospital. These results were confirmed by bacteriocin- and serotyping. We conclude that analysis of these protein patterns--which does not require special reagents - was an adequate method for typing S. marcescens.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0934-8840(89)80037-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anti-interferon gamma-inducible protein 16 antibodies: Identification of a novel autoantigen in idiopathic interstitial pneumonia and its clinical characteristics based on a multicenter cohort study.
Clin Immunol
November 2024
Department of Rheumatology and Clinical Immunology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Autoantibodies are detected in idiopathic interstitial pneumonias (IIPs) without a clear connective tissue disease diagnosis, and their clinical significance is unclear. This study aimed to identify a novel autoantibody in IIPs. We screened 295 IIP patients using a S-methionine labeled protein immunoprecipitation assay.
View Article and Find Full Text PDFScreening and characterization of myositis-related autoantibodies in COVID-19 patients.
Clin Transl Sci
January 2023
Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
Article Synopsis
- - An effective immune response is essential for managing and overcoming COVID-19, but severe cases often show signs of autoimmunity, resembling conditions like inflammatory myopathy and rapidly progressive interstitial lung disease (ILD).
- - In a study of 25 COVID-19 patients with varying symptoms, 36% were found to have autoantibodies related to myositis, with specific antigens identified using line blot analysis, although these antibodies did not correlate with illness severity.
- - To accurately identify and categorize autoantibodies in COVID-19 patients, the study emphasizes using multiple testing methods, as relying on a single method could lead to incorrect conclusions regarding autoimmune disease diagnoses.
In Vivo Analysis of Mitochondrial Protein Synthesis in Saccharomyces cerevisiae Mitochondrial tRNA Mutants.
Methods Mol Biol
July 2022
Department of Biology and Biotechnologies "Charles Darwin", Sapienza University of Rome, Rome, Italy.
I describe here a protocol for the analysis of mitochondrial protein synthesis as a useful tool to characterize the mitochondrial defects associated with mutations in mitochondrial tRNA genes. The yeast Saccharomyces cerevisiae mutants, bearing human equivalent pathogenic mutations, were used as a simple model for analysis. The mitochondrial proteins were labeled by L[S]-methionine incorporation in growing cells, extracted from purified mitochondria, and fractionated by SDS-polyacrylamide gel electrophoresis followed by autoradiography.
View Article and Find Full Text PDFA major checkpoint for protein expression in Rhodobacter sphaeroides during heat stress response occurs at the level of translation.
Environ Microbiol
November 2021
Institute of Microbiology und Molecular Biology, IFZ, Justus-Liebig-Universität, 35292, Giessen, Germany.
Temperature above the physiological optimum is a stress condition frequently faced by bacteria in their natural environments. Here, we were interested in the correlation between levels of RNA and protein under heat stress. Changes in RNA and protein levels were documented in cultures of Rhodobacter sphaeroides using RNA sequencing, quantitative mass spectrometry, western blot analysis, in vivo [ S] methionine-labelling and plasmid-borne reporter fusions.
View Article and Find Full Text PDFCancer in Systemic Sclerosis: Analysis of Antibodies Against Components of the Th/To Complex.
Arthritis Rheumatol
February 2021
Johns Hopkins University, Baltimore, Maryland.
Objective: The aim of this study is to describe 4 of the most common autoantibodies against components of the Th/To complex: human POP1 (hPOP1), RPP25, RPP30, and RPP40. We report their prevalence and clinical characteristics in a systemic sclerosis (SSc) population, and determine whether these specificities are associated with cancer.
Methods: A case-control study was performed using data from the Johns Hopkins Scleroderma Center Cohort.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!