AI Article Synopsis
- Testing the roles of specific microtubule-related proteins in developing neurons is enhanced by methods to effectively deplete these proteins.
- Small interfering RNA (siRNA) is a powerful tool due to its high efficiency in targeting proteins, but it comes with limitations that necessitate careful planning of experiments.
- The authors share valuable insights and experiences from their research using siRNA to explore these functional hypotheses.
Article Abstract
Testing functional hypotheses on the roles played by individual microtubule-related proteins in developing neurons benefits from having an effective means for depleting an individual protein from a population of cultured vertebrate neurons over an appropriate window. Small interfering RNA (siRNA) has the advantage of high transfection efficiency, but has certain limitations that require strategic experimental design. Here we describe the insights that we have gained over the years from using this approach.
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| http://dx.doi.org/10.1016/bs.mcb.2015.06.005 | DOI Listing |
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