AI Article Synopsis
- The study aimed to assess the effectiveness of the new Genedia malaria antigen ELISA test and its combined use with the antibody ELISA test for detecting Plasmodium vivax in blood samples.
- A total of 1,070 samples were examined, including infected patients and healthy volunteers, and results were compared with established testing methods like PCR and microscopy.
- The combined use of antigen and antibody tests resulted in high clinical sensitivity (98.3%) and specificity (97.9%), enhancing the accuracy of diagnosing P. vivax cases in South Korea.
Article Abstract
Objective: To evaluate the clinical sensitivity and specificity of the newly developed Genedia malaria antigen enzyme-linked immunosorbent assay (ELISA) test and to evaluate the diagnostic efficiency of the combined use of the Genedia malaria antigen and antibody ELISA tests to detect Plasmodium vivax in blood samples.
Materials And Methods: In all, 1,070 samples were analyzed: 300 P. vivax-infected patients, 41 samples from posttreatment patients upon follow-up and 729 healthy volunteers. The Genedia malaria antigen ELISA test and the Genedia malaria antibody ELISA 2.0 test were evaluated and compared to polymerase chain reaction and microscopy.
Results: The Genedia malaria antigen ELISA test had a clinical sensitivity of 94.7% (284/300) and a clinical specificity of 99.3% (724/729). The Genedia malaria antibody ELISA 2.0 test had a clinical sensitivity of 94.0% (282/300) and a clinical specificity of 98.4% (717/729). The Genedia malaria antigen ELISA test was able to detect 13 confirmed P. vivax cases without antibodies against P. vivax, whereas the Genedia malaria antibody ELISA 2.0 test detected 11 confirmed P. vivax cases nonreactive to the Genedia malaria antigen ELISA test, and 25 cases from 41 follow-up samples nonreactive in the Genedia malaria antigen ELISA test. The combined Genedia malaria antigen and antibody ELISA 2.0 tests had a clinical sensitivity of 98.3% (295/300) and a clinical specificity of 97.9% (714/729).
Conclusion: The combination of antigen and antibody ELISAs improved the diagnostic sensitivity in P. vivax-confirmed cases in the Republic of Korea.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5588392 | PMC |
| http://dx.doi.org/10.1159/000444144 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combined Use of Malaria Antigen and Antibody Enzyme-Linked Immunosorbent Assay for Blood Screening of Plasmodium vivax in the Republic of Korea.
Med Princ Pract
January 2017
Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul, Republic of Korea.
Article Synopsis
- The study aimed to assess the effectiveness of the new Genedia malaria antigen ELISA test and its combined use with the antibody ELISA test for detecting Plasmodium vivax in blood samples.
- A total of 1,070 samples were examined, including infected patients and healthy volunteers, and results were compared with established testing methods like PCR and microscopy.
- The combined use of antigen and antibody tests resulted in high clinical sensitivity (98.3%) and specificity (97.9%), enhancing the accuracy of diagnosing P. vivax cases in South Korea.
Background: The malaria rapid diagnostic tests (RDTs) are now widely used in the world. Compared to Plasmodium falciparum, a poor sensitivity of RDTs was reported against Plasmodium vivax based on the adopted antibody against pan-Plasmodium antigen lactate dehydrogenase (pLDH) or aldolase. Levels of pLDH were measured from patient with P.
View Article and Find Full Text PDFEvaluation of Plasmodium vivax ELISA for the blood screen.
Trop Med Int Health
December 2010
Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Korea.
Plasmodium vivax malaria is the indigenous strain in the Republic of Korea (ROK). Plasmodium vivax can be transmitted through the transfusions of various blood components, which became a severe problem with the safety of blood transfusions and blood-related products in ROK. We evaluated a P.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!