Background: Methicillin-Resistant Staphylococcus Aureus (MRSA) has been widely recognized as a serious problem in hospital settings. The purpose of this study is to evaluate the predictive value of MRSA colonization factors in the detection of MRSA carriers in an orthopedic ward.
Methods And Materials: A systematic MRSA detection strategy was set up to assess the predictive value of MRSA colonization factors among 554 patients undergoing elective knee arthroplasty.
Results: In total 116 patients were found positive for Staphylococcus Aureus; among those 110/116 patients were found positive for Methicillin-Sensitive Staphylococcus Aureus (MSSA) and 6/116 for MRSA. Only one patient out of six presented two risk factors according to MRSA risk factors. In this study, no correlation was found between the remaining conventional risk factors, according to Belgian guidelines, defined to target high-risk populations and to identify MRSA carriers.
Conclusions: Established criteria for selective MRSA screening do not allow detecting MRSA carriers. The objective of detecting MRSA carriers is not correctly met by the actual applied criteria (Belgian consensus) for a selective screening policy. Future studies should aim at identifying the right risk factors, depending of the country's prevalence of MRSA, to improve the ability to predict the risk of MRSA carriage at hospital admission.
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Science
January 2025
Department of Dermatology, University of Pittsburgh, Pittsburgh, PA, USA.
Itch is a dominant symptom in dermatitis, and scratching promotes cutaneous inflammation, thereby worsening disease. However, the mechanisms through which scratching exacerbates inflammation and whether scratching provides benefit to the host are largely unknown. We found that scratching was required for skin inflammation in mouse models dependent on FcεRI-mediated mast cell activation.
View Article and Find Full Text PDFBraz J Microbiol
January 2025
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector-125, Noida, Uttar Pradesh, 201313, India.
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide due to its antimicrobial resistance. Plant-derived essential oils (EOs) have undergone extensive observational and clinical research to explore their antimicrobial properties. The present study aimed to check mec A positive MRSA isolates using sequencing analysis, determination of chemical composition using gas chromatography-mass spectroscopy (GC-MS), antioxidant, and antimicrobial activity of Anethum graveolens and Piper betle EOs against the infectious agent MRSA.
View Article and Find Full Text PDFNat Prod Res
January 2025
Laboratory of Natural Products and Heterocyclic Synthesis, Department of Organic Chemistry, Faculty of Sciences, The University of Yaoundé 1, Yaoundé, Cameroon.
From the leaves of , fourteen compounds were isolated and identified: D-mannitol (), a mixture of β-sitosterol () and stigmasterol (), α-amyrin (), betulin (), lupeol (), lupenone (), betulinic acid (), taraxerol (), 3β-(E)-coumaroyltaraxerol (), 3β-(Z)-coumaroyltaraxerol (), ursolic acid (), stigmasterol 3-O-β-D-glucoside (), and β-sitosterol 3-O-β-D-glucoside (). These compounds were analysed through NMR spectroscopy (both 1D and 2D) and by comparing them to previously published data. Compounds , , , and - have been identified from this species for the first time.
View Article and Find Full Text PDFMicrobiol Spectr
January 2025
Center for Infectious Diseases, Lab of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands.
Unlabelled: Due to increasing antimicrobial resistance and side effects caused by current standard antimicrobial regimens used for treatment of prosthetic joint infection (PJI), alternative options are urgently needed. We aimed to investigate the effect of clindamycin in different exposure strategies against in an mature biofilm model. In short, 7-day biofilms were generated on polystyrene plates and titanium-aluminum-vanadium discs using a clinical PJI isolate.
View Article and Find Full Text PDFmSystems
January 2025
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Respiratory disease (RD) is a worldwide leading threat to the pig industry, but there is still limited understanding of the pathogens associated with swine RD. In this study, we conducted a nationwide genomic surveillance on identifying viruses, bacteria, and antimicrobial resistance genes (ARGs) from the lungs of pigs with RD in China. By performing metatranscriptomic sequencing combined with metagenomic sequencing, we identified 21 viral species belonging to 12 viral families.
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