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CRISPR/Cas-Based Gene Editing Tools for Large DNA Fragment Integration.
ACS Synth Biol
December 2024
Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
In recent years, gene editing technologies have rapidly evolved to enable precise and efficient genomic modification. These strategies serve as a crucial instrument in advancing our comprehension of genetics and treating genetic disorders. Of particular interest is the manipulation of large DNA fragments, notably the insertion of large fragments, which has emerged as a focal point of research in recent years.
View Article and Find Full Text PDFEfficient genome editing of two-cell mouse embryos via modified CRISPR/Cas electroporation.
Sci Rep
December 2024
Department of Life Innovation, Institute for Biomedical Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.
Creating genetically modified (GM) animals using CRISPR/Cas mediated through the electroporation of two-cell stage embryos, rather than fertilized eggs, holds considerable potential. The full potential of genome editing using two-cell stage embryos is only beginning to be explored. We developed an improved electroporation method to prevent blastomere fusion in two-cell-stage embryos, enabling efficient genome editing.
View Article and Find Full Text PDFBinding of Infectious Hypodermal and Haematopoietic Necrosis Virus-Like Particles to Mannose Receptor Stimulates Antimicrobial Responses in Immune-Related Tissues of Peneaus vannamei.
J Fish Dis
February 2025
School of Medicine, Walailak University, Nakhonsrithammarat, Thailand.
Mannose receptor (MR) is a transmembrane protein and a type of pattern-recognition receptor (PRR) that plays a critical role in the immunity of mammals and fish. In this study, we examined the role of MR in binding with infectious hypodermal and haematopoietic necrosis virus-like particle (IHHN-VLP) and the downstream immune pathway that it triggers in the shrimp Peneaus vannamei. Upon IHHN-VLP challenge, transcripts of MR in P.
View Article and Find Full Text PDFComputing hematopoiesis plasticity in response to genetic mutations and environmental stimulations.
Life Sci Alliance
February 2025
National Key Laboratory of Experimental Hematology, Tianjin, China
Cell plasticity (CP), describing a dynamic cell state, plays a crucial role in maintaining homeostasis during organ morphogenesis, regeneration, and trauma-to-repair biological process. Single-cell-omics datasets provide an unprecedented resource to empower CP analysis. Hematopoiesis offers fertile opportunities to develop quantitative methods for understanding CP.
View Article and Find Full Text PDFInvestigating the Role of Known Arabidopsis Iron Genes in a Stress Resilient Soybean Line.
Int J Mol Sci
October 2024
Agricultural Research Service, Corn Insects, and Crop Genetics Research Unit, United States Department of Agriculture, Ames, IA 50010, USA.
Genes involved in iron deficiency responses have been well characterized in , but their roles in crop species have not been well explored. Reliance on model species may fail to identify novel iron stress mechanisms present within crop species, likely selected by hundreds of years of selection. Fiskeby III (PI 438471) is a soybean line from Sweden that demonstrates high levels of resilience to numerous stresses.
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